Sandbox Reserved 954: Difference between revisions
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Léo Alcapia (talk | contribs) No edit summary |
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[[Image:Structure region.jpg|300px]] [[Image:Fontion3.jpg|400px]] | [[Image:Structure region.jpg|300px]] [[Image:Fontion3.jpg|400px]] | ||
== | ==Function== | ||
===Cysteine proteases mecanism=== | ===Cysteine proteases mecanism=== | ||
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Indeed the reactive center loop (<scene name='60/604473/The_rcl_loop_scene/3'>RCL</scene>) of SCCA1 is very flexible and located away from the center of SCCA1. The inhibitory effect of SCCA1 on the kinase activity of JNK1 is lost when the <scene name='60/604473/The_rcl_loop_scene/3'>RCL</scene> was truncated. Furthermore, a mutant protein created by replacing <scene name='60/604473/Phe_352/1'>one amino-acid</scene> in <scene name='60/604473/The_rcl_loop_scene/3'>RCL</scene> maintain the suppressive activity to JNK1, whereas the inhibitory effect to proteinase is obviously decreased. | Indeed the reactive center loop (<scene name='60/604473/The_rcl_loop_scene/3'>RCL</scene>) of SCCA1 is very flexible and located away from the center of SCCA1. The inhibitory effect of SCCA1 on the kinase activity of JNK1 is lost when the <scene name='60/604473/The_rcl_loop_scene/3'>RCL</scene> was truncated. Furthermore, a mutant protein created by replacing <scene name='60/604473/Phe_352/1'>one amino-acid</scene> in <scene name='60/604473/The_rcl_loop_scene/3'>RCL</scene> maintain the suppressive activity to JNK1, whereas the inhibitory effect to proteinase is obviously decreased. | ||
<ref> PMID : 19166818<ref> | <ref> PMID : 19166818<ref> | ||
This indicate that the exposed <scene name='60/604473/The_rcl_loop_scene/3'>RCL</scene> of the native and mutated forms is essential for the JNK1 inhibitory activity, and the JNK1-interaction site is different from the site of proteinase. Actualy JNK1 may bind the the <scene name='60/604473/The_rcl_loop_scene/3'>RCL</scene> region, except for the proteinase-binding site, or the center of <scene name='60/604473/A_beta_sheet/3'>A β-sheet</scene>. | This indicate that the exposed <scene name='60/604473/The_rcl_loop_scene/3'>RCL</scene> of the native and mutated forms is essential for the JNK1 inhibitory activity, and the JNK1-interaction site is different from the site of proteinase. Actualy JNK1 may bind the the <scene name='60/604473/The_rcl_loop_scene/3'>RCL</scene> region, except for the proteinase-binding site, or the center of <scene name='60/604473/A_beta_sheet/3'>A β-sheet</scene>. http://www.sciencedirect.com/science/article/pii/S0006291X09000953 | ||
http://www.sciencedirect.com/science/article/pii/S0006291X09000953 | |||
===Receptor (LPR) interaction=== | ===Receptor (LPR) interaction=== | ||
