Sandbox Reserved 951: Difference between revisions
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== Global Structure == | == Global Structure == | ||
Luciferase is a 62kDa protein. It contains 550 amino acids. The enzyme can be divide into two domains. On the one hand, the major portion, corresponding to the N-terminal. | Luciferase is a 62kDa protein. It contains 550 amino acids. The enzyme can be divide into two domains. On the one hand, the major portion, corresponding to the N-terminal. On the other hand, the small portion, corresponding to the C-terminal. Those two domains are separated by a large cleft. | ||
The amino acids of C terminal sequence is composed of contiguous residues | ==The C terminal domain== | ||
The amino acids of C terminal sequence is composed of contiguous residues and form a type of lid upon the N terminal domain. It contains two β sheets: one is composed of two short antiparallel strands and the other one is composed of 3 antiparallel strands, mixed with 3 α helices. Those helices are put toward the outside.It is α + β structure. | |||
Each of the two β sheets subdomains (A and B) | ==The N termainal domain== | ||
The amino acids of C terminal sequence is composed of non contiguous residues. It falls into three subdomains (noted A, B, and C).It has an antiparallel β barrel, two β sheets, which are framed by α helices. Each of the two β sheets subdomains (A and B)are composed of 8 β strands and 6 helices. The β sheet A has 5 parallel and 3 antiparallel β strands. The β sheet B has 6 parallel and 2 antiparallel β strands. Those β sheets create a groove, closed on one end by the β barrel. | |||
