4d3l: Difference between revisions

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'''Unreleased structure'''
==SeMet structure of a novel carbohydrate binding module from glycoside hydrolase family 9 (Cel9A) from Ruminococcus flavefaciens FD-1 in the orthorhombic form==
<StructureSection load='4d3l' size='340' side='right' caption='[[4d3l]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4d3l]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D3L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D3L FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PE:NONAETHYLENE+GLYCOL'>2PE</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HHD:(3S)-3-HYDROXYHEPTANEDIOIC+ACID'>HHD</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4v2x|4v2x]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d3l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d3l OCA], [http://pdbe.org/4d3l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4d3l RCSB], [http://www.ebi.ac.uk/pdbsum/4d3l PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Microbial degradation of the plant cell wall is a fundamental biological process with considerable industrial importance. Hydrolysis of recalcitrant polysaccharides is orchestrated by a large repertoire of carbohydrate-active enzymes that display a modular architecture in which a catalytic domain is connected via linker sequences to one or more noncatalytic carbohydrate-binding modules (CBMs). CBMs direct the appended catalytic modules to their target substrates, thus potentiating catalysis. The genome of the most abundant ruminal cellulolytic bacterium, Ruminococcus flavefaciens strain FD-1, provides an opportunity to discover novel cellulosomal proteins involved in plant cell-wall deconstruction. It encodes a modular protein comprising a glycoside hydrolase family 9 catalytic module (GH9) linked to two unclassified tandemly repeated CBMs (termed CBM-Rf6A and CBM-Rf6B) and a C-terminal dockerin. The novel CBM-Rf6A from this protein has been crystallized and data were processed for the native and a selenomethionine derivative to 1.75 and 1.5 A resolution, respectively. The crystals belonged to orthorhombic and cubic space groups, respectively. The structure was solved by a single-wavelength anomalous dispersion experiment using the CCP4 program suite and SHELXC/D/E.


The entry 4d3l is ON HOLD  until Paper Publication
Crystallization and preliminary crystallographic studies of a novel noncatalytic carbohydrate-binding module from the Ruminococcus flavefaciens cellulosome.,Venditto I, Goyal A, Thompson A, Ferreira LM, Fontes CM, Najmudin S Acta Crystallogr F Struct Biol Commun. 2015 Jan 1;71(Pt 1):45-8. doi:, 10.1107/S2053230X14025576. Epub 2015 Jan 1. PMID:25615967<ref>PMID:25615967</ref>


Authors: Venditto, I., Goyal, A., Thompson, A., Ferreira, L.M.A., Fontes, C.M.G.A., Najmudin, S.
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
Description: SeMet structure of a novel carbohydrate binding module from glycoside hydrolase family 9 (Cel9A) from Ruminococcus flavefaciens FD-1 in the orthorhombic form
<div class="pdbe-citations 4d3l" style="background-color:#fffaf0;"></div>
[[Category: Unreleased Structures]]
== References ==
[[Category: Ferreira, L.M.A]]
<references/>
[[Category: Thompson, A]]
__TOC__
</StructureSection>
[[Category: Ferreira, L M.A]]
[[Category: Fontes, C M.G A]]
[[Category: Goyal, A]]
[[Category: Goyal, A]]
[[Category: Najmudin, S]]
[[Category: Najmudin, S]]
[[Category: Thompson, A]]
[[Category: Venditto, I]]
[[Category: Venditto, I]]
[[Category: Fontes, C.M.G.A]]
[[Category: Carbohydrate binding module]]
[[Category: Cel9a]]
[[Category: Cellulosome]]
[[Category: Glycoside hydrolase family 9]]
[[Category: Ruminococcus flavefaciens fd-1]]
[[Category: Sugar binding protein]]
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