4rip: Difference between revisions
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''' | ==BromoUracil substituted structure of intercalation-locked DNA tetraplex== | ||
<StructureSection load='4rip' size='340' side='right' caption='[[4rip]], [[Resolution|resolution]] 2.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4rip]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RIP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RIP FirstGlance]. <br> | |||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BRU:5-BROMO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>BRU</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4rim|4rim]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rip OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rip RCSB], [http://www.ebi.ac.uk/pdbsum/4rip PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
DNA has proved to be an excellent material for nanoscale construction because complementary DNA duplexes are programmable and structurally predictable. However, in the absence of Watson-Crick pairings, DNA can be structurally more diverse. Here, we describe the crystal structures of d(ACTCGGATGAT) and the brominated derivative, d(ACBrUCGGABrUGAT). These oligonucleotides form parallel-stranded duplexes with a crystallographically equivalent strand, resulting in the first examples of DNA crystal structures that contains four different symmetric homo base pairs. Two of the parallel-stranded duplexes are coaxially stacked in opposite directions and locked together to form a tetraplex through intercalation of the 5'-most A-A base pairs between adjacent G-G pairs in the partner duplex. The intercalation region is a new type of DNA tertiary structural motif with similarities to the i-motif. 1H-1H nuclear magnetic resonance and native gel electrophoresis confirmed the formation of a parallel-stranded duplex in solution. Finally, we modified specific nucleotide positions and added d(GAY) motifs to oligonucleotides and were readily able to obtain similar crystals. This suggests that this parallel-stranded DNA structure may be useful in the rational design of DNA crystals and nanostructures. | |||
An intercalation-locked parallel-stranded DNA tetraplex.,Tripathi S, Zhang D, Paukstelis PJ Nucleic Acids Res. 2015 Jan 27. pii: gkv033. PMID:25628357<ref>PMID:25628357</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Paukstelis, P]] | [[Category: Paukstelis, P]] | ||
[[Category: Tripathi, S | [[Category: Tripathi, S K]] | ||
[[Category: Zhang, D]] | [[Category: Zhang, D]] | ||
[[Category: Bromouracil]] | |||
[[Category: Dna]] | |||
[[Category: Homoduplex]] | |||
[[Category: Intercalation-locked dna tetraplex]] | |||
Revision as of 11:53, 12 February 2015
BromoUracil substituted structure of intercalation-locked DNA tetraplex
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