5afe: Difference between revisions
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''' | ==Medium Resolution structure of the C-terminal family 65 Carbohydrate Binding Module (CBM65B) of endoglucanase Cel5A from Eubacterium cellulosolvens with a bound xyloglucan heptasaccharide (XXXG)== | ||
<StructureSection load='5afe' size='340' side='right' caption='[[5afe]], [[Resolution|resolution]] 2.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5afe]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AFE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AFE FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=XYS:XYLOPYRANOSE'>XYS</scene></td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5afe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5afe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5afe RCSB], [http://www.ebi.ac.uk/pdbsum/5afe PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The rumen anaerobic cellulolytic bacterium Eubacterium cellulosolvens produces a large range of cellulases and hemicellulases responsible for the efficient hydrolysis of plant cell wall polysaccharides. One of these enzymes, endoglucanase Cel5A, comprises a tandemly repeated carbohydrate-binding module (CBM65) fused to a glycoside hydrolase family 5 (Cel5A) catalytic domain, joined by flexible linker sequences. The second carbohydrate-binding module located at the C-terminus side of the endoglucanase (CBM65B) has been co-crystallized with either cellohexaose or xyloglucan heptasaccharide. The crystals belong to the hexagonal space group P6(5) and tetragonal space group P4(3)2(1)2, containing a single molecule in the asymmetric unit. The structures of CBM65B have been solved by molecular replacement. | |||
Overproduction, purification, crystallization and preliminary X-ray characterization of the C-terminal family 65 carbohydrate-binding module (CBM65B) of endoglucanase Cel5A from Eubacterium cellulosolvens.,Venditto I, Basle A, Luis AS, Temple MJ, Ferreira LM, Fontes CM, Gilbert HJ, Najmudin S Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Feb 1;69(Pt 2):191-4. doi:, 10.1107/S1744309113001620. Epub 2013 Jan 31. PMID:23385766<ref>PMID:23385766</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
[[Category: | <references/> | ||
[[Category: Gilbert, H | __TOC__ | ||
</StructureSection> | |||
[[Category: Cellulase]] | |||
[[Category: Fontes, C M.G A]] | |||
[[Category: Gilbert, H J]] | |||
[[Category: Najmudin, S]] | [[Category: Najmudin, S]] | ||
[[Category: Venditto, I]] | [[Category: Venditto, I]] | ||
[[Category: | [[Category: Carbohydrate binding module]] | ||
[[Category: Cbm65b]] | |||
[[Category: Endoglucanase cel5a]] | |||
[[Category: Eubacterium cellulosolven]] | |||
[[Category: Hydrolase]] | |||
[[Category: Plant cell wall degradation]] | |||
[[Category: Xylogucan heptasaccharide]] | |||
