Binding site of AChR: Difference between revisions
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== Superimpose HAP on AChBP == | == Superimpose HAP on AChBP == | ||
There is a 13 amino acids high affinity peptide(<scene name='68/688431/Hap/2'>HAP</scene>) which corresponding to residues 187-199 of the AChR that can inhibits the binding of α-BTX to AChR. And the high affinity and specific interaction of α-bungarotoxin (<scene name='68/688431/Structure_of_btx/1'>α-BTX</scene>) with AChR has been of considerable importance in the study of the binding site of AChR.<ref>PMID:11683996</ref> The little peptide can bind to α-BTX as competitive inhibitors of α-BTX biding to AChR. So the complex between α- | There is a 13 amino acids high affinity peptide(<scene name='68/688431/Hap/2'>HAP</scene>) which corresponding to residues 187-199 of the AChR that can inhibits the binding of α-BTX to AChR. And the high affinity and specific interaction of α-bungarotoxin (<scene name='68/688431/Structure_of_btx/1'>α-BTX</scene>) with AChR has been of considerable importance in the study of the binding site of AChR.<ref>PMID:11683996</ref> The little peptide can bind to α-BTX as competitive inhibitors of α-BTX biding to AChR. So the complex between α-BTX and this little peptide(HAP) maybe can used as a model to study the binding site of AChR. | ||
The ligand binding site of AChR is mainly located at the α-subunits. The acetylcholine binding protein(<scene name='68/688431/Achbp/2'>AChBP</scene>) is most closely related to the α-subunits of the nAChR. AChBP is a soluble protein found in the snail [http://en.wikipedia.org/wiki/Lymnaea_stagnalis Lymnaea stagnalis]. Nearly all residues that are conserved within the nAChR family are present in AChBP, including those that are relevant for lignad binding.<ref>PMID:11357122</ref> And AChBP can also bind with α-Neurotoxins. So the AChBP structure is obviously an ideal candidate for testing the relevance of the conformation of the HAP when bound to α-BTX, to that of the corresponding binding region in AChR.<ref>PMID:11683996</ref> | The ligand binding site of AChR is mainly located at the α-subunits. The acetylcholine binding protein(<scene name='68/688431/Achbp/2'>AChBP</scene>) is most closely related to the α-subunits of the nAChR. AChBP is a soluble protein found in the snail [http://en.wikipedia.org/wiki/Lymnaea_stagnalis Lymnaea stagnalis]. Nearly all residues that are conserved within the nAChR family are present in AChBP, including those that are relevant for lignad binding.<ref>PMID:11357122</ref> And AChBP can also bind with α-Neurotoxins. So the AChBP structure is obviously an ideal candidate for testing the relevance of the conformation of the HAP when bound to α-BTX, to that of the corresponding binding region in AChR.<ref>PMID:11683996</ref> | ||
