Sandbox Reserved 993: Difference between revisions
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== Mechanism == | == Mechanism == | ||
It was believed that the chemically produced excited states stemmed from dioxetanone. This idea was proposed based on a common type of chemiluminescence which required O2 at certain points in which dioxetanone is a precursor to the excited state. De Luca and colleagues did a study that proposed that the dioxetanone mechanism for bio- and chemiluminescence were false. Their experiment used oxygen isotopes and concluded that the oxygen atoms that the produced carbon dioxide consisted of did not stem from the consumed oxygen. This study, however, has been analyzed and several flaws have been discovered such as, incomplete chain of events and no proof of CO2 collection from the reaction was obtainable. It was stated that the CO2 produced was pumped directly out of the reaction. This was not possible due to the high reaction rate of CO2 and tert-butoxide ion and the stability of monoalkyl carbonates. Johnson and Shimomura determined that an oxygen atom that makes up the CO2 does indeed stem from the O2 consumed by the reaction in firefly bioluminescence. De Luca and colleagues reevaluated their work and their results agreed with Johnson and Shimomura. Therefore, the dioxetane-dioxetanone mechanism for firefly bioluminescence and chemiluminescence is supported.(Chemi- and Bioluminescence of Firefly Luciferin) | |||
Step one: In the photinus pyralis, the reaction begins with luciferin. Luciferase catalyzes ATP and magnesium ion to produce luciferyl AMP from luciferin. | |||
Step 2: Luciferase then catalyzes O2, producing light and oxyluciferin from Luciferyl AMP. (Chemi- and Bioluminescence of Firefly Luciferin) (Firefly luciferase in chemical biology: A compendium of inhibitors, mechanistic evaluation of chemotypes, and suggested use as a reporter) | |||
== Structure == | == Structure == | ||
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</StructureSection> | </StructureSection> | ||
== References == | == References == | ||
1. White E. H., Steinmetz M. G., Miano J. D., Wildes P. D. and Morland R. (1980) "Chemi- and bioluminescence of firefly luciferin", J. Am. Chem. Soc. 102(9): 3199-3208. | |||
2. Thorne N., Shen M., Lea W. A., Simeonov A., Lovell S., Auld D. S. and Inglese J. (2012) "Firefly luciferase in chemical biology: A compendium of inhibitor, mechanistic evaluation of chemotypes, and suggested use as a reporter", Chem. Biol. 19(8): 1060-1072. doi:http://dx.doi.org/10.1016%2Fj.chembiol.2012.07.015 | |||
<references/> | <references/> | ||
