Sandbox Reserved 987: Difference between revisions
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You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue. | You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue. | ||
== | == Background == | ||
Cocaine Esterase (CocE) is the most efficient protein to hydrolyze the cocaine domain known to date ''in vivo''.<sup><ref>"Effects of cocaine esterase following its repeated administration with cocaine in mice" Mei-Chaun Ko, Diwahar Narasimhan, Aaron A. Berlin, Nicholas W. Lukacs, Roger K. Sunahara, James H. Woods. Drug and Alcohol Dependence 1 May 2009;101:202-09. doi: 10.1016/j.drugalcdep.2009.01.002</ref></sup> Cocaine Esterase is used in bacterium ''Rhodococcus'' which hydrolyzes the cocaine that it uptakes and uses it for carbons and nitrogens. Although this protein metabolizes cocaine in bacterium, it is sure to induce an immune response as it is foreign to the human body. This could mitigate the effects of CocE if a person had suffered from cocaine toxicity. | Cocaine Esterase (CocE) is the most efficient protein to hydrolyze the cocaine domain known to date ''in vivo''.<sup><ref>"Effects of cocaine esterase following its repeated administration with cocaine in mice" Mei-Chaun Ko, Diwahar Narasimhan, Aaron A. Berlin, Nicholas W. Lukacs, Roger K. Sunahara, James H. Woods. Drug and Alcohol Dependence 1 May 2009;101:202-09. doi: 10.1016/j.drugalcdep.2009.01.002</ref></sup> Cocaine Esterase is used in bacterium ''Rhodococcus'' which hydrolyzes the cocaine that it uptakes and uses it for carbons and nitrogens. Although this protein metabolizes cocaine in bacterium, it is sure to induce an immune response as it is foreign to the human body. This could mitigate the effects of CocE if a person had suffered from cocaine toxicity. | ||
== Structure == | == Structure == | ||
<scene name='69/691529/Cocaine_esterase/1'>TextToBeDisplayed</scene> | <scene name='69/691529/Cocaine_esterase/1'>TextToBeDisplayed</scene> | ||
== | |||
== Function == | |||
Cocaine esterase is used to catalyze the following reaction: | Cocaine esterase is used to catalyze the following reaction: | ||
cocaine + H<sub>2</sub>O ←→ ecgonine methyl ester + benzoate <sup><ref>Cocaine esterase | cocaine + H<sub>2</sub>O ←→ ecgonine methyl ester + benzoate <sup><ref>Cocaine esterase | ||
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Reaction mechanism for cocaine esterase-catalyzed hydrolyses of (+)- and (-)-cocaine: unexpected common rate-determining step. | Reaction mechanism for cocaine esterase-catalyzed hydrolyses of (+)- and (-)-cocaine: unexpected common rate-determining step. | ||
Liu J1, Zhao X, Yang W, Zhan CG. | Liu J1, Zhao X, Yang W, Zhan CG. | ||
== | |||
== Medical Relevance == | |||
This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. | This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. | ||
