UBC13 MMS2: Difference between revisions
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== Regulation == | == Regulation == | ||
The regulation of | The regulation of Ubc13 is controlled by the competitive binding of the tow different UEV's, Mms2 and UEV1A. Ubc13 binding to Mms2 activates the DNA repair pathway, while Ubc13 binding to UEV1A activates the NF-kappaB pathway, a gene regulation pathway involved in DNA transcription factors. These two opposing pathways being downstream targets for Ubc13-bound complexes is the basis for regulation. | ||
==Coordinating Enzymes== | ==Coordinating Enzymes== | ||
* Another E2 complex, Rad6-Rad18, starts the process of DNA repair by monoubiquinating PCNA near the replication fork of DNA. This DNA repair will arrest cell cycle progression until DNA repair is complete. | * Another E2 complex, Rad6-Rad18, starts the process of DNA repair by monoubiquinating PCNA near the replication fork of DNA. This DNA repair will arrest cell cycle progression until DNA repair is complete. | ||
* UEV1A, another cofactor enzyme that binds to | * UEV1A, another cofactor enzyme that binds to Ubc13, is thought to compete with Mms2 for binding to Ubc13. This is thought to be a regulatory mechanism for Ubc13 activity in the nucleus of cells. | ||
*Several DNA polymerases such as rev1, pol eta, and pol zeta contain Ubiquitin-binding domains that recognize K164 polyubiquitination of PCNA. | *Several DNA polymerases such as rev1, pol eta, and pol zeta contain Ubiquitin-binding domains that recognize K164 polyubiquitination of PCNA. | ||
*Rad5, an E3 RING (really interesting new gene) protein, interacts with the | *Rad5, an E3 RING (really interesting new gene) protein, interacts with the Ubc13-Mms2 heterodimer in order to ligate the Ubiquitin on the PCNA. Rad5, as well as Rad18 (RING proteins) are involved in the recruitment of Ubc13-Mms2 heterodimer formation. | ||
== Pathway for DNA Repair == | == Pathway for DNA Repair == | ||
