UBC13 MMS2: Difference between revisions
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==Summary== | ==Summary== | ||
Ubc13 is an E2 ubiquitin conjugating enzyme that can form a heterodimer with Mms2 to function as a part of the Translesion DNA Synthesis Pathway (TLS). When bound to Mms2, Ubc13 will polyubiquinate PCNA, Proliferating Cell Nuclear Antigen, a sliding clamp protein at the DNA transcription fork. Ubc13-Mms2 functions to | Ubc13 is an E2 ubiquitin conjugating enzyme that can form a heterodimer with Mms2 to function as a part of the Translesion DNA Synthesis Pathway (TLS). When bound to Mms2, Ubc13 will polyubiquinate PCNA, Proliferating Cell Nuclear Antigen, a sliding clamp protein at the DNA transcription fork. Ubc13-Mms2 functions to polyubiquitinate PCNA following the initial monoubiquitination by Rad6-Rad18(another E2 complex). | ||
== Function == | == Function == | ||
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==Coordinating Enzymes== | ==Coordinating Enzymes== | ||
* Another E2 complex, Rad6-Rad18, starts the process of DNA repair by | * Another E2 complex, Rad6-Rad18, starts the process of DNA repair by monoubiquitinating PCNA near the replication fork of DNA. This DNA repair will arrest cell cycle progression until DNA repair is complete. | ||
* UEV1A, another cofactor enzyme that binds to Ubc13, is thought to compete with Mms2 for binding to Ubc13. This is thought to be a regulatory mechanism for Ubc13 activity in the nucleus of cells. | * UEV1A, another cofactor enzyme that binds to Ubc13, is thought to compete with Mms2 for binding to Ubc13. This is thought to be a regulatory mechanism for Ubc13 activity in the nucleus of cells. | ||
*Several DNA polymerases such as rev1, pol eta, and pol zeta contain Ubiquitin-binding domains that recognize K164 polyubiquitination of PCNA | *Several DNA polymerases such as rev1, pol eta, and pol zeta contain Ubiquitin-binding domains that recognize K164 polyubiquitination of PCNA. | ||
*Rad5, an E3 RING ( | *Rad5, an E3 RING (Really Interesting New Gene) protein, interacts with the Ubc13-Mms2 heterodimer in order to ligate the ubiquitin on the PCNA. Rad5, as well as Rad18 (RING proteins) are involved in the recruitment of Ubc13-Mms2 heterodimer formation. | ||
== Pathway for DNA Repair == | == Pathway for DNA Repair == | ||
