UBC13 MMS2: Difference between revisions
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Ubc13 weights 17.6 kDA, and Mms2 is 16.8 kDA. The heterodimer is stable at high stalt concentrations (1 M), suggesting strong interactions between the two. Kd between the Ubc13 and Mms2 is 2 uM. | Ubc13 weights 17.6 kDA, and Mms2 is 16.8 kDA. The heterodimer is stable at high stalt concentrations (1 M), suggesting strong interactions between the two. Kd between the Ubc13 and Mms2 is 2 uM. | ||
Phe57 and Glu55 of Ubc13 interact with the N-terminal domain of Mms2 to ensure stable docking. Additionally, Arg70 hydrophobically interacts with an alpha helix of Mms2 in two places. Mms2’s Phe13 is inserted between Glu55, Phe57, and Arg70 of Ubc13 to create a hydrophobic pocket. It is therorized that Glu55 and Arg70 of Ubc13 are more important for recognition instead of stability | Phe57 and Glu55 of Ubc13 interact with the N-terminal domain of Mms2 to ensure stable docking. Additionally, Arg70 hydrophobically interacts with an alpha helix of Mms2 in two places. Mms2’s Phe13 is inserted between Glu55, Phe57, and Arg70 of Ubc13 to create a hydrophobic pocket. It is therorized that Glu55 and Arg70 of Ubc13 are more important for recognition instead of stability. | ||
==Mechanism== | ==Mechanism== | ||
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== References == | == References == | ||
<references/> | <references/> | ||
