UBC13 MMS2: Difference between revisions

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Ubc13 weights 17.6 kDA, and Mms2 is 16.8 kDA<ref name=Pastushok> Pastushok, L.; FAU, M. T.; FAU, E. M.; Xiao, W. A single Mms2 "key" residue insertion into a Ubc13 pocket determines the interface specificity of a human Lys63 ubiquitin conjugation complex. The Journal of biological chemistry JID - 2985121R 0902.</ref>. The heterodimer is stable at high stalt concentrations (1 M), suggesting strong interactions between the two. Kd between the Ubc13 and Mms2 is 2 uM.

Phe57 and Glu55 of Ubc13 interact with the N-terminal domain of Mms2 to ensure stable docking<ref name=Pastushok> Pastushok, L.; FAU, M. T.; FAU, E. M.; Xiao, W. A single Mms2 "key" residue insertion into a Ubc13 pocket determines the interface specificity of a human Lys63 ubiquitin conjugation complex. The Journal of biological chemistry JID - 2985121R 0902.</ref>. Additionally, Arg70 hydrophobically interacts with an alpha helix of Mms2 in two places~~</ref>~~<ref name=Pastushok> 7. Pastushok, L.; FAU, M. T.; FAU, E. M.; Xiao, W. A single Mms2 "key" residue insertion into a Ubc13 pocket determines the interface specificity of a human Lys63 ubiquitin conjugation complex. The Journal of biological chemistry JID - 2985121R 0902.</ref>. Mms2’s Phe13 is inserted between Glu55, Phe57, and Arg70 of Ubc13 to create a hydrophobic pocket</ref><ref name=Pastushok> 7. Pastushok, L.; FAU, M. T.; FAU, E. M.; Xiao, W. A single Mms2 "key" residue insertion into a Ubc13 pocket determines the interface specificity of a human Lys63 ubiquitin conjugation complex. The Journal of biological chemistry JID - 2985121R 0902.</ref>. It is therorized that Glu55 and Arg70 of Ubc13 are more important for recognition instead of stability</ref><ref name=Pastushok> 7. Pastushok, L.; FAU, M. T.; FAU, E. M.; Xiao, W. A single Mms2 "key" residue insertion into a Ubc13 pocket determines the interface specificity of a human Lys63 ubiquitin conjugation complex. The Journal of biological chemistry JID - 2985121R 0902.</ref>.

Phe57 and Glu55 of Ubc13 interact with the N-terminal domain of Mms2 to ensure stable docking<ref name=Pastushok> Pastushok, L.; FAU, M. T.; FAU, E. M.; Xiao, W. A single Mms2 "key" residue insertion into a Ubc13 pocket determines the interface specificity of a human Lys63 ubiquitin conjugation complex. The Journal of biological chemistry JID - 2985121R 0902.</ref>. Additionally, Arg70 hydrophobically interacts with an alpha helix of Mms2 in two places<ref name=Pastushok> 7. Pastushok, L.; FAU, M. T.; FAU, E. M.; Xiao, W. A single Mms2 "key" residue insertion into a Ubc13 pocket determines the interface specificity of a human Lys63 ubiquitin conjugation complex. The Journal of biological chemistry JID - 2985121R 0902.</ref>. Mms2’s Phe13 is inserted between Glu55, Phe57, and Arg70 of Ubc13 to create a hydrophobic pocket</ref><ref name=Pastushok> 7. Pastushok, L.; FAU, M. T.; FAU, E. M.; Xiao, W. A single Mms2 "key" residue insertion into a Ubc13 pocket determines the interface specificity of a human Lys63 ubiquitin conjugation complex. The Journal of biological chemistry JID - 2985121R 0902.</ref>. It is therorized that Glu55 and Arg70 of Ubc13 are more important for recognition instead of stability</ref><ref name=Pastushok> 7. Pastushok, L.; FAU, M. T.; FAU, E. M.; Xiao, W. A single Mms2 "key" residue insertion into a Ubc13 pocket determines the interface specificity of a human Lys63 ubiquitin conjugation complex. The Journal of biological chemistry JID - 2985121R 0902.</ref>.

==Mechanism==

UBC13 MMS2: Difference between revisions

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@@ Line 30: / Line 30: @@
 Ubc13 weights 17.6 kDA, and Mms2 is 16.8 kDA<ref name=Pastushok> Pastushok, L.; FAU, M. T.; FAU, E. M.; Xiao, W. A single Mms2 "key" residue insertion into a Ubc13 pocket determines the interface specificity of a human Lys63 ubiquitin conjugation complex. The Journal of biological chemistry JID - 2985121R 0902.</ref>. The heterodimer is stable at high stalt concentrations (1 M), suggesting strong interactions between the two. Kd between the Ubc13 and Mms2 is 2 uM.
-Phe57 and Glu55 of Ubc13 interact with the N-terminal domain of Mms2 to ensure stable docking<ref name=Pastushok> Pastushok, L.; FAU, M. T.; FAU, E. M.; Xiao, W. A single Mms2 "key" residue insertion into a Ubc13 pocket determines the interface specificity of a human Lys63 ubiquitin conjugation complex. The Journal of biological chemistry JID - 2985121R 0902.</ref>. Additionally, Arg70 hydrophobically interacts with an alpha helix of Mms2 in two places</ref><ref name=Pastushok> 7. Pastushok, L.; FAU, M. T.; FAU, E. M.; Xiao, W. A single Mms2 "key" residue insertion into a Ubc13 pocket determines the interface specificity of a human Lys63 ubiquitin conjugation complex. The Journal of biological chemistry JID - 2985121R 0902.</ref>. Mms2’s Phe13 is inserted between Glu55, Phe57, and Arg70 of Ubc13 to create a hydrophobic pocket</ref><ref name=Pastushok> 7. Pastushok, L.; FAU, M. T.; FAU, E. M.; Xiao, W. A single Mms2 "key" residue insertion into a Ubc13 pocket determines the interface specificity of a human Lys63 ubiquitin conjugation complex. The Journal of biological chemistry JID - 2985121R 0902.</ref>. It is therorized that Glu55 and Arg70 of Ubc13 are more important for recognition instead of stability</ref><ref name=Pastushok> 7. Pastushok, L.; FAU, M. T.; FAU, E. M.; Xiao, W. A single Mms2 "key" residue insertion into a Ubc13 pocket determines the interface specificity of a human Lys63 ubiquitin conjugation complex. The Journal of biological chemistry JID - 2985121R 0902.</ref>.
+Phe57 and Glu55 of Ubc13 interact with the N-terminal domain of Mms2 to ensure stable docking<ref name=Pastushok> Pastushok, L.; FAU, M. T.; FAU, E. M.; Xiao, W. A single Mms2 "key" residue insertion into a Ubc13 pocket determines the interface specificity of a human Lys63 ubiquitin conjugation complex. The Journal of biological chemistry JID - 2985121R 0902.</ref>. Additionally, Arg70 hydrophobically interacts with an alpha helix of Mms2 in two places<ref name=Pastushok> 7. Pastushok, L.; FAU, M. T.; FAU, E. M.; Xiao, W. A single Mms2 "key" residue insertion into a Ubc13 pocket determines the interface specificity of a human Lys63 ubiquitin conjugation complex. The Journal of biological chemistry JID - 2985121R 0902.</ref>. Mms2’s Phe13 is inserted between Glu55, Phe57, and Arg70 of Ubc13 to create a hydrophobic pocket</ref><ref name=Pastushok> 7. Pastushok, L.; FAU, M. T.; FAU, E. M.; Xiao, W. A single Mms2 "key" residue insertion into a Ubc13 pocket determines the interface specificity of a human Lys63 ubiquitin conjugation complex. The Journal of biological chemistry JID - 2985121R 0902.</ref>. It is therorized that Glu55 and Arg70 of Ubc13 are more important for recognition instead of stability</ref><ref name=Pastushok> 7. Pastushok, L.; FAU, M. T.; FAU, E. M.; Xiao, W. A single Mms2 "key" residue insertion into a Ubc13 pocket determines the interface specificity of a human Lys63 ubiquitin conjugation complex. The Journal of biological chemistry JID - 2985121R 0902.</ref>.
 ==Mechanism==