Sandbox Reserved 993: Difference between revisions
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The active site environment influences the wavelength of the light emitted. Single amino acid changes within the active site of ''Photinus pyralis'' luciferase can shift the luminescence from yellow-green to red. Modifying the position of the Ser314-Leu319 loop near the active site can alter Biolumanescence color. When assayed under acidic conditions, all spectra underwent a red shift while basic conditions caused a blue shift. These experiments were done using ''E. coli'' as the host organism indicating that the internal pH of the cell was close to the external pH. These findings suggest a possible use of bioluminescence in pH monitoring, biosensing and tissue and animal imaging.<ref name=Shapiro2005 /> | The active site environment influences the wavelength of the light emitted. Single amino acid changes within the active site of ''Photinus pyralis'' luciferase can shift the luminescence from yellow-green to red. Modifying the position of the Ser314-Leu319 loop near the active site can alter Biolumanescence color. When assayed under acidic conditions, all spectra underwent a red shift while basic conditions caused a blue shift. These experiments were done using ''E. coli'' as the host organism indicating that the internal pH of the cell was close to the external pH. These findings suggest a possible use of bioluminescence in pH monitoring, biosensing and tissue and animal imaging.<ref name=Shapiro2005 /> | ||
== Function == | == Function == | ||
There are three main functions of bioluminescence in nature: offense, defense and communication. Offense suggests baiting or enticing prey, defense suggests camouflage or protection and communication relates to courtship and mating. The literature suggests that the firefly species mainly use bioluminescence for communication purposes.<ref name=Greer2002> Greer, L.F., and Szalay, A.A. (2002). “Imaging of Light Emission from the Expression of Luciferases in Living Cells and Organisms: A Reivew.” Luminescence 17(1):43-74. doi: 10.1002/bio.676. <ref/>== Lab Use == | There are three main functions of bioluminescence in nature: offense, defense and communication. Offense suggests baiting or enticing prey, defense suggests camouflage or protection and communication relates to courtship and mating. The literature suggests that the firefly species mainly use bioluminescence for communication purposes.<ref name=Greer2002> Greer, L.F., and Szalay, A.A. (2002). “Imaging of Light Emission from the Expression of Luciferases in Living Cells and Organisms: A Reivew.” Luminescence 17(1):43-74. doi: 10.1002/bio.676. <ref/> | ||
== Lab Use == | |||
This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. | This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. | ||
