4xbm

X-ray crystal structure of Notch ligand Delta-like 1

spinqualitylabelsall models 4xbm, resolution 3.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4xbm is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: FUC Resources: FirstGlance, OCA, RCSB, PDBsum Disease [DLL1_HUMAN] Septopreoptic holoprosencephaly;Semilobar holoprosencephaly;Lobar holoprosencephaly;Alobar holoprosencephaly;Midline interhemispheric variant of holoprosencephaly;Microform holoprosencephaly. Function [DLL1_HUMAN] Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. Publication Abstract from PubMed The Notch pathway is a fundamental signalling system in most multicellular animals. We have determined the X-ray crystal structure of the extracellular domain of the Notch ligand Delta-like ligand-1 (Dll-1). This is the first structure of a Delta-like ligand. The structure incorporates the N-terminal C2 domain, receptor-binding DSL domain, and the first six (of eight) EGF-like repeats, which form a highly extended conformation, confirmed by analytical ultracentrifugation. Comparison of our structure with a fragment of Jagged1 ligand allows us to dissect the similarities and differences between the ligand families. Differences in the C2 domains of Dll-1 and Jagged1 suggest their lipid binding properties are likely to differ. A conserved hydrophobic patch on the surface of both Dll-1 and Jagged1 provides a likely receptor-interaction site that is common to both ligands. We also explore the binding affinity of Dll-1 for a fragment of Notch1 using different techniques. Apparent binding affinities vary when different techniques are used, explaining discrepancies in the literature. Using analytical ultracentrifugation, we perform for the first time binding analyses where both receptor and ligand are in solution, which confirms a Kd of 10muM for this interaction. Notch ligand Delta-like1: X-ray crystal structure and binding affinity.,Kershaw NJ, Church NL, Griffin MD, Luo CS, Adams TE, Burgess AW Biochem J. 2015 Feb 26. PMID:25715738 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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