Sandbox Reserved 993: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
Kelsey Hodge (talk | contribs) No edit summary |
||
| Line 18: | Line 18: | ||
Step 2: Luciferase then catalyzes O<sub>2</sub>, producing light, CO<sub>2</sub>, and oxyluciferin from Luciferyl AMP.<ref name=White1980 /><ref name=Thorne2012>Thorne, N., Shen, M., Lea, W. A., Simeonov, A., Lovell, S., Auld, D. S. and Inglese, J. (2012) "Firefly luciferase in chemical biology: A compendium of inhibitor, mechanistic evaluation of chemotypes, and suggested use as a reporter", Chem. Biol. 19(8): 1060-1072. doi:http://dx.doi.org/10.1016%2Fj.chembiol.2012.07.015</ref> | Step 2: Luciferase then catalyzes O<sub>2</sub>, producing light, CO<sub>2</sub>, and oxyluciferin from Luciferyl AMP.<ref name=White1980 /><ref name=Thorne2012>Thorne, N., Shen, M., Lea, W. A., Simeonov, A., Lovell, S., Auld, D. S. and Inglese, J. (2012) "Firefly luciferase in chemical biology: A compendium of inhibitor, mechanistic evaluation of chemotypes, and suggested use as a reporter", Chem. Biol. 19(8): 1060-1072. doi:http://dx.doi.org/10.1016%2Fj.chembiol.2012.07.015</ref> | ||
[[Image: | [[Image:Luciferin_Mechanism.jpg]] | ||
The active site environment influences the wavelength of the light emitted. Single amino acid changes within the active site of ''Photinus pyralis'' luciferase can shift the luminescence from yellow-green to red. Modifying the position of the Ser314-Leu319 loop near the active site can alter bioluminescence color. When assayed under acidic conditions, all spectra underwent a red shift, while basic conditions caused a blue shift. These experiments were done using ''E. coli'' as the host organism indicating that the internal pH of the cell was close to the external pH. These findings suggest a possible use of bioluminescence in pH monitoring, biosensing, tissue and animal imaging.<ref name=Shapiro2005 /> | The active site environment influences the wavelength of the light emitted. Single amino acid changes within the active site of ''Photinus pyralis'' luciferase can shift the luminescence from yellow-green to red. Modifying the position of the Ser314-Leu319 loop near the active site can alter bioluminescence color. When assayed under acidic conditions, all spectra underwent a red shift, while basic conditions caused a blue shift. These experiments were done using ''E. coli'' as the host organism indicating that the internal pH of the cell was close to the external pH. These findings suggest a possible use of bioluminescence in pH monitoring, biosensing, tissue and animal imaging.<ref name=Shapiro2005 /> | ||
