2ra5: Difference between revisions
No edit summary |
No edit summary |
||
| Line 4: | Line 4: | ||
|PDB= 2ra5 |SIZE=350|CAPTION= <scene name='initialview01'>2ra5</scene>, resolution 2.40Å | |PDB= 2ra5 |SIZE=350|CAPTION= <scene name='initialview01'>2ra5</scene>, resolution 2.40Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand= | |LIGAND= <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SRT:S,R+MESO-TARTARIC+ACID'>SRT</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= SCO6256 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2 Bacteria]) | |GENE= SCO6256 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2 Bacteria]) | ||
|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam07702 UTRA], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG2188 PhnF], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=smart00345 HTH_GNTR]</span> | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ra5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ra5 OCA], [http://www.ebi.ac.uk/pdbsum/2ra5 PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=2ra5 RCSB]</span> | |||
}} | }} | ||
| Line 29: | Line 31: | ||
[[Category: Xu, X.]] | [[Category: Xu, X.]] | ||
[[Category: Zheng, H.]] | [[Category: Zheng, H.]] | ||
[[Category: beta structure]] | [[Category: beta structure]] | ||
[[Category: dna-binding]] | [[Category: dna-binding]] | ||
| Line 42: | Line 42: | ||
[[Category: utra domain]] | [[Category: utra domain]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 06:45:39 2008'' | ||
Revision as of 04:45, 26 March 2008
| |||||||||||||
| 2ra5, resolution 2.40Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | IPA, MSE, SRT | ||||||||||||
| Gene: | SCO6256 (Bacteria) | ||||||||||||
| Domains: | UTRA, PhnF, HTH_GNTR | ||||||||||||
| Resources: | FirstGlance, OCA, PDBsum, JenaLib, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of the putative transcriptional regulator from Streptomyces coelicolor
Overview
We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.
About this Structure
2RA5 is a Single protein structure of sequence from Bacteria. Full crystallographic information is available from OCA.
Reference
In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4. PMID:17982461
Page seeded by OCA on Wed Mar 26 06:45:39 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Bacteria
- Single protein
- Edwards, A M.
- Joachimiak, A.
- Kim, Y.
- MCSG, Midwest Center for Structural Genomics.
- Savchenko, A.
- Xu, X.
- Zheng, H.
- Beta structure
- Dna-binding
- Mcsg
- Midwest center for structural genomic
- Protein structure initiative
- Psi-2
- Structural genomic
- Transcription
- Transcription regulation
- Utra domain
