4yeb: Difference between revisions

Line 1:

'''~~Unreleased~~ structure'''

==Structural characterization of a synaptic adhesion complex==

== Structural highlights ==

<table><tr><td colspan='2'>[[4yeb]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YEB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YEB FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4rmk|4rmk]], [[4rml|4rml]]</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yeb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yeb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4yeb RCSB], [http://www.ebi.ac.uk/pdbsum/4yeb PDBsum]</span></td></tr>

</table>

== Function ==

[[http://www.uniprot.org/uniprot/LPHN3_MOUSE LPHN3_MOUSE]] May be involved in the development of glutamatergic synapses in the cortex. Important in determining the connectivity rates between the principal neurons in the cortex.<ref>PMID:24739570</ref>

== Publication Abstract from PubMed ==

Latrophilins (LPHNs) are adhesion-like G-protein-coupled receptors implicated in attention-deficit/hyperactivity disorder. Recently, LPHN3 was found to regulate excitatory synapse number through trans interactions with fibronectin leucine-rich repeat transmembrane 3 (FLRT3). By isothermal titration calorimetry, we determined that only the olfactomedin (OLF) domain of LPHN3 is necessary for FLRT3 association. By multi-crystal native single-wavelength anomalous diffraction phasing, we determined the crystal structure of the OLF domain. This structure is a five-bladed beta propeller with a Ca2+ ion bound in the central pore, which is capped by a mobile loop that allows the ion to exchange with the solvent. The crystal structure of the OLF/FLRT3 complex shows that LPHN3-OLF in the closed state binds with high affinity to the concave face of FLRT3-LRR with a combination of hydrophobic and charged residues. Our study provides structural and functional insights into the molecular mechanism underlying the contribution of LPHN3/FLRT3 to the development of glutamatergic synapses.

~~The entry 4yeb is ON HOLD until Paper Publication~~

Structural and Mechanistic Insights into the Latrophilin3-FLRT3 Complex that Mediates Glutamatergic Synapse Development.,Ranaivoson FM, Liu Q, Martini F, Bergami F, von Daake S, Li S, Lee D, Demeler B, Hendrickson WA, Comoletti D Structure. 2015 Jul 28. pii: S0969-2126(15)00275-0. doi:, 10.1016/j.str.2015.06.022. PMID:26235031<ref>PMID:26235031</ref>

~~Authors: Ranaivoson~~, ~~F.M., Liu, Q., Martini, F., Bergami, F~~.~~, von Daake,~~ S.~~, Li, S., Lee, D., Demeler, B., Hendrickson, W.A., Comoletti, D~~.

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

~~Description: Structural characterization of a synaptic adhesion complex~~

== References ==

~~[[Category: Unreleased Structures]]~~

[[Category: Li, S]]

__TOC__

</StructureSection>

[[Category: Bergami, F]]

[[Category: Comoletti, D]]

[[Category: ~~Martini~~, F]]

[[Category: Daake, S von]]

[[Category: Demeler, B]]

[[Category: ~~Bergami~~, ~~F]]~~

[[Category: Hendrickson, W A]]

~~[[Category: Von Daake, S~~]]

[[Category: Lee, D]]

[[Category: Li, S]]

[[Category: Liu, Q]]

[[Category: ~~Hendrickson~~, ~~W.A~~]]

[[Category: Martini, F]]

[[Category: Ranaivoson, F.M]]

[[Category: Ranaivoson, F M]]

[[Category: Central nervous system]]

[[Category: Complex]]

[[Category: Flrt3]]

[[Category: Latrophilin 3]]

[[Category: Signaling protein]]

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+==Structural characterization of a synaptic adhesion complex==
+<StructureSection load='4yeb' size='340' side='right' caption='[[4yeb]], [[Resolution|resolution]] 3.19&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4yeb]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YEB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YEB FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4rmk|4rmk]], [[4rml|4rml]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yeb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yeb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4yeb RCSB], [http://www.ebi.ac.uk/pdbsum/4yeb PDBsum]</span></td></tr>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/LPHN3_MOUSE LPHN3_MOUSE]] May be involved in the development of glutamatergic synapses in the cortex. Important in determining the connectivity rates between the principal neurons in the cortex.<ref>PMID:24739570</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Latrophilins (LPHNs) are adhesion-like G-protein-coupled receptors implicated in attention-deficit/hyperactivity disorder. Recently, LPHN3 was found to regulate excitatory synapse number through trans interactions with fibronectin leucine-rich repeat transmembrane 3 (FLRT3). By isothermal titration calorimetry, we determined that only the olfactomedin (OLF) domain of LPHN3 is necessary for FLRT3 association. By multi-crystal native single-wavelength anomalous diffraction phasing, we determined the crystal structure of the OLF domain. This structure is a five-bladed beta propeller with a Ca2+ ion bound in the central pore, which is capped by a mobile loop that allows the ion to exchange with the solvent. The crystal structure of the OLF/FLRT3 complex shows that LPHN3-OLF in the closed state binds with high affinity to the concave face of FLRT3-LRR with a combination of hydrophobic and charged residues. Our study provides structural and functional insights into the molecular mechanism underlying the contribution of LPHN3/FLRT3 to the development of glutamatergic synapses.
-The entry 4yeb is ON HOLD  until Paper Publication
+Structural and Mechanistic Insights into the Latrophilin3-FLRT3 Complex that Mediates Glutamatergic Synapse Development.,Ranaivoson FM, Liu Q, Martini F, Bergami F, von Daake S, Li S, Lee D, Demeler B, Hendrickson WA, Comoletti D Structure. 2015 Jul 28. pii: S0969-2126(15)00275-0. doi:, 10.1016/j.str.2015.06.022. PMID:26235031<ref>PMID:26235031</ref>
-Authors: Ranaivoson, F.M., Liu, Q., Martini, F., Bergami, F., von Daake, S., Li, S., Lee, D., Demeler, B., Hendrickson, W.A., Comoletti, D.
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-Description: Structural characterization of a synaptic adhesion complex
+== References ==
-[[Category: Unreleased Structures]]
+<references/>
-[[Category: Li, S]]
+__TOC__
+</StructureSection>
+[[Category: Bergami, F]]
 [[Category: Comoletti, D]]
-[[Category: Martini, F]]
+[[Category: Daake, S von]]
 [[Category: Demeler, B]]
-[[Category: Bergami, F]]
+[[Category: Hendrickson, W A]]
-[[Category: Von Daake, S]]
 [[Category: Lee, D]]
+[[Category: Li, S]]
 [[Category: Liu, Q]]
-[[Category: Hendrickson, W.A]]
+[[Category: Martini, F]]
-[[Category: Ranaivoson, F.M]]
+[[Category: Ranaivoson, F M]]
+[[Category: Central nervous system]]
+[[Category: Complex]]
+[[Category: Flrt3]]
+[[Category: Latrophilin 3]]
+[[Category: Signaling protein]]