Sandbox Reserved 1058: Difference between revisions
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==Mechanism of Action== | ==Mechanism of Action== | ||
[[Image:TCA_Cycle.png|500 px|left|thumb|'''Figure 5. Citric Acid Cycle with Glyoxylate Shunt Pathway.''' In several bacterial species, there is a carbon conserving gloxylate shunt pathway that converts isocitrate to malate in two steps instead of the usual five steps.]] | [[Image:TCA_Cycle.png|500 px|left|thumb|'''Figure 5. Citric Acid Cycle with Glyoxylate Shunt Pathway.''' In several bacterial species, there is a carbon conserving gloxylate shunt pathway that converts isocitrate to malate in two steps instead of the usual five steps.]] | ||
[[Image: | [[Image:Complete_Mechanism.png|250 px|left|thumb|'''Figure 6. Observed Mechanism for the Breakdown of Isocitrate by Isocitrate Lyase.''' His193 shifts the pKa of Cys191 and removes its proton. This allows Cys191 to extract a proton from the hydroxyl group of isocitrate. The resulting oxyanion forms a carbonyl and forces the lysis of a C-C bond. Glyoxylate and the enol form of succinate are formed and stabilized with a Mg<sup>2+</sup> ion. The succinate enolate resonates and extracts the proton back from Cys191 to form succinate.]] | ||
==Disease Association== | ==Disease Association== | ||
===Clinical Implications=== | ===Clinical Implications=== | ||
