2mwz: Difference between revisions
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''' | ==A structure of G-quadruplex== | ||
<StructureSection load='2mwz' size='340' side='right' caption='[[2mwz]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2mwz]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MWZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MWZ FirstGlance]. <br> | |||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=3ZO:2-DEOXY-XANTHOSINE-5-MONOPHOSPHATE'>3ZO</scene>, <scene name='pdbligand=8OG:8-OXO-2-DEOXY-GUANOSINE-5-MONOPHOSPHATE'>8OG</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2gku|2gku]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mwz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mwz OCA], [http://pdbe.org/2mwz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mwz RCSB], [http://www.ebi.ac.uk/pdbsum/2mwz PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
G-quadruplexes are four-stranded structures built from stacked G-tetrads (G.G.G.G), which are planar cyclical assemblies of four guanine bases interacting through Hoogsteen hydrogen bonds. A G-quadruplex containing a single guanine analog substitution, such as 8-oxoguanine (O) or xanthine (X), would suffer from a loss of a Hoogsteen hydrogen bond within a G-tetrad and/or potential steric hindrance. We show that a proper arrangement of O and X bases can reestablish the hydrogen-bond pattern within a G.G.X.O tetrad. Rational incorporation of G.G.X.O tetrads in a (3+1) G-quadruplex demonstrated a similar folding topology and thermal stability to that of the unmodified G-quadruplex. pH titration conducted on X.O-modified G-quadruplexes indicated a protonation-deprotonation equilibrium of X with a pKa approximately 6.7. The solution structure of a G-quadruplex containing a G.G.X.O tetrad was determined, displaying the same folding topology in both the protonated and deprotonated states. A G-quadruplex containing a deprotonated X.O pair was shown to exhibit a more electronegative groove compared to that of the unmodified one. These differences are likely to manifest in the electronic properties of G-quadruplexes and may have important implications for drug targeting and DNA-protein interactions. | |||
Xanthine and 8-oxoguanine in G-quadruplexes: formation of a G.G.X.O tetrad.,Cheong VV, Heddi B, Lech CJ, Phan AT Nucleic Acids Res. 2015 Sep 22. pii: gkv826. PMID:26400177<ref>PMID:26400177</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2mwz" style="background-color:#fffaf0;"></div> | |||
[[Category: | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Cheong, V V]] | |||
[[Category: Heddi, B]] | [[Category: Heddi, B]] | ||
[[Category: | [[Category: Lech, C J]] | ||
[[Category: Phan, A | [[Category: Phan, A T]] | ||
[[Category: | [[Category: Dna]] | ||
[[Category: Xanthosine]] | |||
Revision as of 03:48, 16 October 2015
A structure of G-quadruplex
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