4zgo: Difference between revisions
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''' | ==Structure of C-terminally truncated Cdc123 from Schizosaccharomyces pombe== | ||
<StructureSection load='4zgo' size='340' side='right' caption='[[4zgo]], [[Resolution|resolution]] 2.06Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4zgo]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZGO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZGO FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zgo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zgo OCA], [http://pdbe.org/4zgo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zgo RCSB], [http://www.ebi.ac.uk/pdbsum/4zgo PDBsum]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/CD123_SCHPO CD123_SCHPO]] Regulates the cell cycle in a nutrient dependent manner. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Eukaryotic initiation factor 2 (eIF2), a heterotrimeric guanosine triphosphatase, has a central role in protein biosynthesis by supplying methionylated initiator tRNA to the ribosomal translation initiation complex and by serving as a target for translational control in response to stress. Recent work identified a novel step indispensable for eIF2 function: assembly of eIF2 from its three subunits by the cell proliferation protein Cdc123. We report the first crystal structure of a Cdc123 representative, that from Schizosaccharomyces pombe, both isolated and bound to domain III of Saccharomyces cerevisiae eIF2gamma. The structures show that Cdc123 resembles enzymes of the ATP-grasp family. Indeed, Cdc123 binds ATP-Mg(2+), and conserved residues contacting ATP-Mg(2+) are essential for Cdc123 to support eIF2 assembly and cell viability. A docking of eIF2alphagamma onto Cdc123, combined with genetic and biochemical experiments, allows us to propose a model explaining how Cdc123 participates in the biogenesis of eIF2 through facilitating assembly of eIF2gamma to eIF2alpha. | |||
Cdc123, a Cell Cycle Regulator Needed for eIF2 Assembly, Is an ATP-Grasp Protein with Unique Features.,Panvert M, Dubiez E, Arnold L, Perez J, Mechulam Y, Seufert W, Schmitt E Structure. 2015 Sep 1;23(9):1596-608. doi: 10.1016/j.str.2015.06.014. Epub 2015, Jul 23. PMID:26211610<ref>PMID:26211610</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4zgo" style="background-color:#fffaf0;"></div> | |||
== References == | |||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
</StructureSection> | |||
[[Category: Arnold, L]] | |||
[[Category: Dubiez, E]] | |||
[[Category: Mechulam, Y]] | [[Category: Mechulam, Y]] | ||
[[Category: Panvert, M]] | [[Category: Panvert, M]] | ||
[[Category: | [[Category: Perez, J]] | ||
[[Category: Schmitt, E]] | |||
[[Category: Seufert, W]] | [[Category: Seufert, W]] | ||
[[Category: Atp-grap fold]] | |||
[[Category: Cell cycle]] | |||
[[Category: Eif2 assembly]] | |||
Revision as of 14:31, 30 September 2015
Structure of C-terminally truncated Cdc123 from Schizosaccharomyces pombe
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