4zk7: Difference between revisions

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'''Unreleased structure'''
==Crystal structure of rescued two-component self-assembling tetrahedral cage T33-31==
<StructureSection load='4zk7' size='340' side='right' caption='[[4zk7]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4zk7]] is a 24 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZK7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZK7 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zk7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4zk7 RCSB], [http://www.ebi.ac.uk/pdbsum/4zk7 PDBsum]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/CUTA_THET8 CUTA_THET8]] Involved in resistance toward heavy metals (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We recently reported the development of a computational method for the design of co-assembling, multi-component protein nanomaterials. While four such materials were validated at high-resolution by X-ray crystallography, low yield of soluble protein prevented X-ray structure determination of a fifth designed material, T33-09. Here we report the design and crystal structure of T33-31, a variant of T33-09 with improved soluble yield resulting from redesign efforts focused on mutating solvent-exposed side chains to charged amino acids. The structure is found to match the computational design model with atomic-level accuracy, providing further validation of the design approach and demonstrating a simple and potentially general means of improving the yield of designed protein nanomaterials. This article is protected by copyright. All rights reserved.


The entry 4zk7 is ON HOLD  until Paper Publication
Structure of a designed tetrahedral protein assembly variant engineered to have improved soluble expression.,Bale JB, Park RU, Liu Y, Gonen S, Gonen T, Cascio D, King NP, Yeates TO, Baker D Protein Sci. 2015 Jul 15. doi: 10.1002/pro.2748. PMID:26174163<ref>PMID:26174163</ref>


Authors: Liu, Y., Cascio, D., Sawaya, M.R., Bale, J., Collazo, M.J., Park, R., King, N., Baker, D., Yeates, T.
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
Description: Crystal structure of rescued two-component self-assembling tetrahedral cage T33-31
== References ==
[[Category: Unreleased Structures]]
<references/>
__TOC__
</StructureSection>
[[Category: Baker, D]]
[[Category: Bale, J]]
[[Category: Bale, J]]
[[Category: Sawaya, M.R]]
[[Category: Cascio, D]]
[[Category: Collazo, M J]]
[[Category: King, N]]
[[Category: Liu, Y]]
[[Category: Liu, Y]]
[[Category: Baker, D]]
[[Category: Park, R]]
[[Category: Park, R]]
[[Category: King, N]]
[[Category: Sawaya, M R]]
[[Category: Yeates, T]]
[[Category: Yeates, T]]
[[Category: Cascio, D]]
[[Category: Computational design]]
[[Category: Collazo, M.J]]
[[Category: Nanomaterial]]
[[Category: Protein binding]]
[[Category: Rosetta]]
[[Category: Self-assembly]]
[[Category: Solubility]]
[[Category: Symmetric]]
[[Category: Tetrahedral]]
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