4zr0: Difference between revisions

Revision as of 14:52, 29 July 2015

Full length scs7p (only hydroxylase domain visible)

Structural highlights

4zr0 is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	ZN
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[SCS7_YEAST] Ceramide hydroxylase involved in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids. Hydroxylates the very long chain fatty acid of ceramides at C2 and C3.^[1] ^[2] ^[3] ^[4] ^[5] ^[6]

References

↑ Swain E, Baudry K, Stukey J, McDonough V, Germann M, Nickels JT Jr. Sterol-dependent regulation of sphingolipid metabolism in Saccharomyces cerevisiae. J Biol Chem. 2002 Jul 19;277(29):26177-84. Epub 2002 May 10. PMID:12006573 doi:https://dx.doi.org/10.1074/jbc.M204115200
↑ Guan XL, Wenk MR. Mass spectrometry-based profiling of phospholipids and sphingolipids in extracts from Saccharomyces cerevisiae. Yeast. 2006 Apr 30;23(6):465-77. PMID:16652392 doi:https://dx.doi.org/10.1002/yea.1362
↑ Bosson R, Guillas I, Vionnet C, Roubaty C, Conzelmann A. Incorporation of ceramides into Saccharomyces cerevisiae glycosylphosphatidylinositol-anchored proteins can be monitored in vitro. Eukaryot Cell. 2009 Mar;8(3):306-14. doi: 10.1128/EC.00257-08. Epub 2008 Dec 12. PMID:19074599 doi:https://dx.doi.org/10.1128/EC.00257-08
↑ Mitchell AG, Martin CE. Fah1p, a Saccharomyces cerevisiae cytochrome b5 fusion protein, and its Arabidopsis thaliana homolog that lacks the cytochrome b5 domain both function in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids. J Biol Chem. 1997 Nov 7;272(45):28281-8. PMID:9353282
↑ Haak D, Gable K, Beeler T, Dunn T. Hydroxylation of Saccharomyces cerevisiae ceramides requires Sur2p and Scs7p. J Biol Chem. 1997 Nov 21;272(47):29704-10. PMID:9368039
↑ Dunn TM, Haak D, Monaghan E, Beeler TJ. Synthesis of monohydroxylated inositolphosphorylceramide (IPC-C) in Saccharomyces cerevisiae requires Scs7p, a protein with both a cytochrome b5-like domain and a hydroxylase/desaturase domain. Yeast. 1998 Mar 15;14(4):311-21. PMID:9559540 doi:<311::AID-YEA220>3.0.CO;2-B https://dx.doi.org/10.1002/(SICI)1097-0061(19980315)14:4<311::AID-YEA220>3.0.CO;2-B

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OCA

[1] Swain E, Baudry K, Stukey J, McDonough V, Germann M, Nickels JT Jr. Sterol-dependent regulation of sphingolipid metabolism in Saccharomyces cerevisiae. J Biol Chem. 2002 Jul 19;277(29):26177-84. Epub 2002 May 10. PMID:12006573 doi:https://dx.doi.org/10.1074/jbc.M204115200

[2] Guan XL, Wenk MR. Mass spectrometry-based profiling of phospholipids and sphingolipids in extracts from Saccharomyces cerevisiae. Yeast. 2006 Apr 30;23(6):465-77. PMID:16652392 doi:https://dx.doi.org/10.1002/yea.1362

[3] Bosson R, Guillas I, Vionnet C, Roubaty C, Conzelmann A. Incorporation of ceramides into Saccharomyces cerevisiae glycosylphosphatidylinositol-anchored proteins can be monitored in vitro. Eukaryot Cell. 2009 Mar;8(3):306-14. doi: 10.1128/EC.00257-08. Epub 2008 Dec 12. PMID:19074599 doi:https://dx.doi.org/10.1128/EC.00257-08

[4] Mitchell AG, Martin CE. Fah1p, a Saccharomyces cerevisiae cytochrome b5 fusion protein, and its Arabidopsis thaliana homolog that lacks the cytochrome b5 domain both function in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids. J Biol Chem. 1997 Nov 7;272(45):28281-8. PMID:9353282

[5] Haak D, Gable K, Beeler T, Dunn T. Hydroxylation of Saccharomyces cerevisiae ceramides requires Sur2p and Scs7p. J Biol Chem. 1997 Nov 21;272(47):29704-10. PMID:9368039

[6] Dunn TM, Haak D, Monaghan E, Beeler TJ. Synthesis of monohydroxylated inositolphosphorylceramide (IPC-C) in Saccharomyces cerevisiae requires Scs7p, a protein with both a cytochrome b5-like domain and a hydroxylase/desaturase domain. Yeast. 1998 Mar 15;14(4):311-21. PMID:9559540 doi:<311::AID-YEA220>3.0.CO;2-B https://dx.doi.org/10.1002/(SICI)1097-0061(19980315)14:4<311::AID-YEA220>3.0.CO;2-B

[1]

[2]

[3]

[4]

[5]

[6]

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+==Full length scs7p (only hydroxylase domain visible)==
+<StructureSection load='4zr0' size='340' side='right' caption='[[4zr0]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
-The entry 4zr0 is ON HOLD
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4zr0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZR0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZR0 FirstGlance]. <br>
-Authors: Zhu, G., Koszelak-Rosenblum, M., Malkowski, M.G., Membrane Protein Structural Biology Consortium (MPSBC)
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zr0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zr0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4zr0 RCSB], [http://www.ebi.ac.uk/pdbsum/4zr0 PDBsum]</span></td></tr>
-Description: Full length scs7p (only hydroxylase domain visible)
+</table>
-[[Category: Unreleased Structures]]
+== Function ==
+[[http://www.uniprot.org/uniprot/SCS7_YEAST SCS7_YEAST]] Ceramide hydroxylase involved in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids. Hydroxylates the very long chain fatty acid of ceramides at C2 and C3.<ref>PMID:12006573</ref> <ref>PMID:16652392</ref> <ref>PMID:19074599</ref> <ref>PMID:9353282</ref> <ref>PMID:9368039</ref> <ref>PMID:9559540</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Koszelak-Rosenblum, M]]
-[[Category: Membrane Protein Structural Biology Consortium (Mpsbc)]]
+[[Category: MPSBC, Membrane Protein Structural Biology Consortium]]
+[[Category: Malkowski, M G]]
 [[Category: Zhu, G]]
-[[Category: Malkowski, M.G]]
+[[Category: Fa2h]]
+[[Category: Fah1p]]
+[[Category: Fatty acid hydroxylase]]
+[[Category: Membrane protein structural biology consortium]]
+[[Category: Mpsbc]]
+[[Category: Oxidoreductase]]
+[[Category: Psi-biology]]
+[[Category: Scs7p]]
+[[Category: Structural genomic]]

4zr0: Difference between revisions

Revision as of 14:52, 29 July 2015

Full length scs7p (only hydroxylase domain visible)

Structural highlights

Function

References

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