359d: Difference between revisions
No edit summary |
No edit summary |
||
| Line 4: | Line 4: | ||
|PDB= 359d |SIZE=350|CAPTION= <scene name='initialview01'>359d</scene>, resolution 2.900Å | |PDB= 359d |SIZE=350|CAPTION= <scene name='initialview01'>359d</scene>, resolution 2.900Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=TB:TERBIUM(III) ION'>TB</scene> | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=TB:TERBIUM(III)+ION'>TB</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=359d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=359d OCA], [http://www.ebi.ac.uk/pdbsum/359d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=359d RCSB]</span> | |||
}} | }} | ||
| Line 24: | Line 27: | ||
[[Category: Scott, W G.]] | [[Category: Scott, W G.]] | ||
[[Category: Uhlenbeck, O C.]] | [[Category: Uhlenbeck, O C.]] | ||
[[Category: catalytic rna]] | [[Category: catalytic rna]] | ||
[[Category: loop]] | [[Category: loop]] | ||
[[Category: rna hammerhead ribozyme]] | [[Category: rna hammerhead ribozyme]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:21:42 2008'' | ||
Revision as of 02:21, 31 March 2008
| |||||||||||||
| 359d, resolution 2.900Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | A, C, G, TB, U | ||||||||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
INHIBITION OF THE HAMMERHEAD RIBOZYME CLEAVAGE REACTION BY SITE-SPECIFIC BINDING OF TB(III)
Overview
Terbium(III) [Tb(III)] was shown to inhibit the hammerhead ribozyme by competing with a single magnesium(II) ion. X-ray crystallography revealed that the Tb(III) ion binds to a site adjacent to an essential guanosine in the catalytic core of the ribozyme, approximately 10 angstroms from the cleavage site. Synthetic modifications near this binding site yielded an RNA substrate that was resistant to Tb(III) binding and capable of being cleaved, even in the presence of up to 20 micromolar Tb(III). It is suggested that the magnesium(II) ion thought to bind at this site may act as a switch, affecting the conformational changes required to achieve the transition state.
About this Structure
359D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Inhibition of the hammerhead ribozyme cleavage reaction by site-specific binding of Tb., Feig AL, Scott WG, Uhlenbeck OC, Science. 1998 Jan 2;279(5347):81-4. PMID:9417029
Page seeded by OCA on Mon Mar 31 05:21:42 2008
