3b4b: Difference between revisions
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|PDB= 3b4b |SIZE=350|CAPTION= <scene name='initialview01'>3b4b</scene>, resolution 2.7Å | |PDB= 3b4b |SIZE=350|CAPTION= <scene name='initialview01'>3b4b</scene>, resolution 2.7Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=GLP:GLUCOSAMINE+6-PHOSPHATE'>GLP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=3AD:3 | |LIGAND= <scene name='pdbligand=GLP:GLUCOSAMINE+6-PHOSPHATE'>GLP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=3AD:3'-DEOXYADENOSINE'>3AD</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
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[[Category: rna]] | [[Category: rna]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 15:58:16 2008'' | ||
Revision as of 13:58, 23 March 2008
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| 3b4b, resolution 2.7Å | |||||||||||||
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| Ligands: | GLP, MG and 3AD | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
T. tengcongensis glmS ribozyme with G40A mutation, bound to glucosamine-6-phosphate and a substrate RNA with a 2'5'-phosphodiester linkage
Overview
The glmS ribozyme is a catalytic riboswitch that is activated for endonucleolytic cleavage by the coenzyme glucosamine-6-phosphate. Using kinetic assays and X-ray crystallography, we identify an active-site mutation of a conserved guanine that abolishes catalysis without perturbing coenzyme binding. Our results provide evidence that coenzyme function requires a specific nucleobase to interact with the nucleophile of the cleavage reaction.
About this Structure
3B4B is a Protein complex structure of sequences from Thermoanaerobacter tengcongensis. Full crystallographic information is available from OCA.
Reference
Essential role of an active-site guanine in glmS ribozyme catalysis., Klein DJ, Been MD, Ferre-D'Amare AR, J Am Chem Soc. 2007 Dec 5;129(48):14858-9. Epub 2007 Nov 9. PMID:17990888
Page seeded by OCA on Sun Mar 23 15:58:16 2008
