3jab: Difference between revisions
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''' | ==Domain organization and conformational plasticity of the G protein effector, PDE6== | ||
<StructureSection load='3jab' size='340' side='right' caption='[[3jab]], [[Resolution|resolution]] 11.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3jab]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JAB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3JAB FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IBM:3-ISOBUTYL-1-METHYLXANTHINE'>IBM</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3jab FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jab OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3jab RCSB], [http://www.ebi.ac.uk/pdbsum/3jab PDBsum]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/CNRG_BOVIN CNRG_BOVIN]] Participates in processes of transmission and amplification of the visual signal. cGMP-PDEs are the effector molecules in G-protein-mediated phototransduction in vertebrate rods and cones. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The cGMP phosphodiesterase of rod photoreceptor cells, PDE6, is the key effector enzyme in phototransduction. Two large catalytic subunits, PDE6alpha and -beta, each contain one catalytic domain and two non-catalytic GAF domains, whereas two small inhibitory PDE6gamma subunits allow tight regulation by the G protein transducin. The structure of holo-PDE6 in complex with the ROS-1 antibody Fab fragment was determined by cryo-electron microscopy. The approximately 11 A map revealed previously unseen features of PDE6, and each domain was readily fit with high resolution structures. A structure of PDE6 in complex with prenyl-binding protein (PrBP/delta) indicated the location of the PDE6 C-terminal prenylations. Reconstructions of complexes with Fab fragments bound to N or C termini of PDE6gamma revealed that PDE6gamma stretches from the catalytic domain at one end of the holoenzyme to the GAF-A domain at the other. Removal of PDE6gamma caused dramatic structural rearrangements, which were reversed upon its restoration. | |||
Domain Organization and Conformational Plasticity of the G Protein Effector, PDE6.,Zhang Z, He F, Constantine R, Baker ML, Baehr W, Schmid MF, Wensel TG, Agosto MA J Biol Chem. 2015 May 15;290(20):12833-43. doi: 10.1074/jbc.M115.647636. Epub, 2015 Mar 25. PMID:25809480<ref>PMID:25809480</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
[[Category: | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bos taurus]] | |||
[[Category: Mus musculus]] | |||
[[Category: Agosto, M A]] | |||
[[Category: Baehr, W]] | |||
[[Category: Baker, M L]] | |||
[[Category: Constantine, R]] | |||
[[Category: He, F]] | [[Category: He, F]] | ||
[[Category: Schmid, M F]] | |||
[[Category: Wensel, T G]] | |||
[[Category: Zhang, Z]] | [[Category: Zhang, Z]] | ||
[[Category: | [[Category: Hydrolase-immune system complex]] | ||
[[Category: | [[Category: Phosphodiesterase]] | ||
[[Category: | [[Category: Photoreceptor]] | ||
Revision as of 14:00, 10 June 2015
Domain organization and conformational plasticity of the G protein effector, PDE6
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