Intrinsically Disordered Protein: Difference between revisions
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The yeast transcriptional activator GCN4 belongs to a large family of eukaryotic transcription factors including Fos, Jun and CREB. All family members have a [[DNA]] recognition motif consists of a coiled-coil dimerization element, the leucine-zipper, and an adjoining basic region, which mediates DNA binding. This basic region is largely unstructured in the absence of DNA, addition of DNA containing a GCN4 binding site induce the transition of this region from unstructured to α-helical. | The yeast transcriptional activator GCN4 belongs to a large family of eukaryotic transcription factors including Fos, Jun and CREB. All family members have a [[DNA]] recognition motif consists of a coiled-coil dimerization element, the leucine-zipper, and an adjoining basic region, which mediates DNA binding. This basic region is largely unstructured in the absence of DNA, addition of DNA containing a GCN4 binding site induce the transition of this region from unstructured to α-helical. | ||
{{Clear}} | {{Clear}} | ||
== Practical Implications of IUPs == | == Practical Implications of IUPs == | ||
There is evidence that large intrinsically unstructured regions interferes with crystallization<ref name="IDSG">PMID: 23232152</ref>. Oldfield ''et al.'', 2013<ref name="IDSG" />, concluded: | There is evidence that large intrinsically unstructured regions interferes with crystallization<ref name="IDSG">PMID: 23232152</ref>. Oldfield ''et al.'', 2013<ref name="IDSG" />, concluded: | ||
<blockquote> | <blockquote> | ||
The limited amount of intrinsic disorder present as missing density regions agrees with the idea that intrinsically disordered regions, particularly long disordered regions, inhibits successful determination of crystal structures, and suggests that avoiding or tailoring disordered proteins may aid in the determination of crystal structures. | The limited amount of intrinsic disorder present as missing density regions agrees with the idea that intrinsically disordered regions, particularly long disordered regions, inhibits successful determination of crystal structures, and suggests that avoiding or tailoring disordered proteins may aid in the determination of crystal structures. | ||
</blockquote> | </blockquote> | ||
</StructureSection> | |||
__NOTOC__ | |||
== References and Notes == | == References and Notes == | ||
<small><references /></small> | <small><references /></small> | ||
