3boy: Difference between revisions
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|PDB= 3boy |SIZE=350|CAPTION= <scene name='initialview01'>3boy</scene>, resolution 1.70Å | |PDB= 3boy |SIZE=350|CAPTION= <scene name='initialview01'>3boy</scene>, resolution 1.70Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=HIS:HISTIDINE'>HIS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= hutP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis]) | |GENE= hutP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis]) | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1vea|1VEA]], [[1wpt|1WPT]], [[1wmq|1WMQ]], [[1wpu|1WPU]], [[1wrq|1WRQ]], [[1wpv|1WPV]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3boy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3boy OCA], [http://www.ebi.ac.uk/pdbsum/3boy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3boy RCSB]</span> | |||
}} | }} | ||
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[[Category: Shinkai, A.]] | [[Category: Shinkai, A.]] | ||
[[Category: Yokoyama, S.]] | [[Category: Yokoyama, S.]] | ||
[[Category: activator]] | [[Category: activator]] | ||
[[Category: anti-termination]] | [[Category: anti-termination]] | ||
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[[Category: transcription/rna complex]] | [[Category: transcription/rna complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:27:58 2008'' | ||
Revision as of 02:27, 31 March 2008
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| 3boy, resolution 1.70Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | A, G, HIS, MG, U | ||||||||||||
| Gene: | hutP (Bacillus subtilis) | ||||||||||||
| Related: | 1VEA, 1WPT, 1WMQ, 1WPU, 1WRQ, 1WPV
| ||||||||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of the HutP antitermination complex bound to the HUT mRNA
Overview
HutP is an L-histidine-activated RNA binding protein that regulates the expression of the histidine utilization (hut) operon in Bacillus subtilis by binding to cis-acting regulatory sequences on the hut mRNA. The crystal structure of HutP complexed with an L-histidine analog showed a novel fold; there are four antiparallel beta strands in the central region of each monomer, with two alpha helices each on the front and back. Two HutP monomers form a dimer, and three dimers are arranged in crystallographic 3-fold symmetry to form a hexamer. A histidine analog was located in between the two monomers of HutP, with the imidazole group of L-histidine hydrogen bonded to Glu81. An activation mechanism is proposed based on the identification of key residues of HutP. The HutP binding region in hut mRNA was defined: it consists of three UAG trinucleotide motifs separated by four spacer nucleotides. Residues of HutP potentially important for RNA binding were identified.
About this Structure
3BOY is a Protein complex structure of sequences from Bacillus subtilis. This structure supersedes the now removed PDB entry 2GZT. Full crystallographic information is available from OCA.
Reference
Crystal structure of activated HutP; an RNA binding protein that regulates transcription of the hut operon in Bacillus subtilis., Kumarevel T, Fujimoto Z, Karthe P, Oda M, Mizuno H, Kumar PK, Structure. 2004 Jul;12(7):1269-80. PMID:15242603
Page seeded by OCA on Mon Mar 31 05:27:58 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Bacillus subtilis
- Protein complex
- Balasundaresan, D.
- Jeyakanthan, J.
- Kumar, P K.R.
- Kumarevel, T S.
- RSGI, RIKEN Structural Genomics/Proteomics Initiative.
- Shinkai, A.
- Yokoyama, S.
- Activator
- Anti-termination
- Histidine metabolism
- Hutp
- Hutp-rna complex
- National project on protein structural and functional analyse
- Nppsfa
- Riken structural genomics/proteomics initiative
- Rna-binding
- Rsgi
- Structural genomic
- Transcription regulation
- Transcription/rna complex
