1ct5: Difference between revisions
No edit summary |
No edit summary |
||
| Line 4: | Line 4: | ||
|PDB= 1ct5 |SIZE=350|CAPTION= <scene name='initialview01'>1ct5</scene>, resolution 2.00Å | |PDB= 1ct5 |SIZE=350|CAPTION= <scene name='initialview01'>1ct5</scene>, resolution 2.00Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=PLP:PYRIDOXAL-5'-PHOSPHATE'>PLP</scene> | |LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5'-PHOSPHATE'>PLP</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd00635 YBL036c_PLPDEIII]</span> | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ct5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ct5 OCA], [http://www.ebi.ac.uk/pdbsum/1ct5 PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=1ct5 RCSB]</span> | |||
}} | }} | ||
| Line 26: | Line 28: | ||
[[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]] | [[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]] | ||
[[Category: Swaminathan, S.]] | [[Category: Swaminathan, S.]] | ||
[[Category: mad]] | [[Category: mad]] | ||
[[Category: new york structural genomix research consortium]] | [[Category: new york structural genomix research consortium]] | ||
| Line 38: | Line 39: | ||
[[Category: yeast]] | [[Category: yeast]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 05:51:15 2008'' | ||
Revision as of 03:51, 26 March 2008
| |||||||||||||
| 1ct5, resolution 2.00Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | MSE, PLP | ||||||||||||
| Domains: | YBL036c_PLPDEIII | ||||||||||||
| Resources: | FirstGlance, OCA, PDBsum, JenaLib, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
CRYSTAL STRUCTURE OF YEAST HYPOTHETICAL PROTEIN YBL036C-SELENOMET CRYSTAL
Overview
Yeast hypothetical protein YBL036C (SWISS-PROT P38197), initially thought to be a member of an 11-protein family, was selected for crystal structure determination since no structural or functional information was available. The structure has been determined independently by MIR and MAD methods to 2.0 A resolution. The MAD structure was determined largely through automated model building. The protein folds as a TIM barrel beginning with a long N-terminal helix, in contrast to the classic triose phosphate isomerase (TIM) structure, which begins with a beta-strand. A cofactor, pyridoxal 5'-phosphate, is covalently bound near the C-terminal end of the barrel, the usual active site in TIM-barrel folds. A single-domain monomeric molecule, this yeast protein resembles the N-terminal domain of alanine racemase or ornithine decarboxylase, both of which are two-domain dimeric proteins. The yeast protein has been shown to have amino-acid racemase activity. Although selected as a member of a protein family having no obvious relationship to proteins of known structure, the protein fold turned out to be a well known and widely distributed fold. This points to the need for a more comprehensive base of structural information and better structure-modeling tools before the goal of structure prediction from amino-acid sequences can be realised. In this case, similarity to a known structure allowed inferences to be made about the structure and function of a widely distributed protein family.
About this Structure
1CT5 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structure of a yeast hypothetical protein selected by a structural genomics approach., Eswaramoorthy S, Gerchman S, Graziano V, Kycia H, Studier FW, Swaminathan S, Acta Crystallogr D Biol Crystallogr. 2003 Jan;59(Pt 1):127-35. Epub 2002, Dec 19. PMID:12499548
Page seeded by OCA on Wed Mar 26 05:51:15 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Saccharomyces cerevisiae
- Single protein
- Burley, S K.
- Eswaramoorthy, S.
- NYSGXRC, New York Structural GenomiX Research Consortium.
- Swaminathan, S.
- Mad
- New york structural genomix research consortium
- Nysgxrc
- Protein structure initiative
- Psi
- Pyridoxal-5'-phosphate
- Selenomethionine
- Structural genomic
- Tim barrel
- Yeast
