5d5v: Difference between revisions

Revision as of 19:38, 30 December 2015

Crystal structure of human Hsf1 with Satellite III repeat DNA

Structural highlights

5d5v is a 4 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	MG
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[HSF1_HUMAN] DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7] ^[8]

References

↑ Holmberg CI, Hietakangas V, Mikhailov A, Rantanen JO, Kallio M, Meinander A, Hellman J, Morrice N, MacKintosh C, Morimoto RI, Eriksson JE, Sistonen L. Phosphorylation of serine 230 promotes inducible transcriptional activity of heat shock factor 1. EMBO J. 2001 Jul 16;20(14):3800-10. PMID:11447121 doi:https://dx.doi.org/10.1093/emboj/20.14.3800
↑ Knauf U, Newton EM, Kyriakis J, Kingston RE. Repression of human heat shock factor 1 activity at control temperature by phosphorylation. Genes Dev. 1996 Nov 1;10(21):2782-93. PMID:8946918
↑ Kline MP, Morimoto RI. Repression of the heat shock factor 1 transcriptional activation domain is modulated by constitutive phosphorylation. Mol Cell Biol. 1997 Apr;17(4):2107-15. PMID:9121459
↑ Xia W, Guo Y, Vilaboa N, Zuo J, Voellmy R. Transcriptional activation of heat shock factor HSF1 probed by phosphopeptide analysis of factor 32P-labeled in vivo. J Biol Chem. 1998 Apr 10;273(15):8749-55. PMID:9535852
↑ Guo Y, Guettouche T, Fenna M, Boellmann F, Pratt WB, Toft DO, Smith DF, Voellmy R. Evidence for a mechanism of repression of heat shock factor 1 transcriptional activity by a multichaperone complex. J Biol Chem. 2001 Dec 7;276(49):45791-9. Epub 2001 Oct 2. PMID:11583998 doi:https://dx.doi.org/10.1074/jbc.M105931200
↑ Soncin F, Zhang X, Chu B, Wang X, Asea A, Ann Stevenson M, Sacks DB, Calderwood SK. Transcriptional activity and DNA binding of heat shock factor-1 involve phosphorylation on threonine 142 by CK2. Biochem Biophys Res Commun. 2003 Apr 4;303(2):700-6. PMID:12659875
↑ Hietakangas V, Ahlskog JK, Jakobsson AM, Hellesuo M, Sahlberg NM, Holmberg CI, Mikhailov A, Palvimo JJ, Pirkkala L, Sistonen L. Phosphorylation of serine 303 is a prerequisite for the stress-inducible SUMO modification of heat shock factor 1. Mol Cell Biol. 2003 Apr;23(8):2953-68. PMID:12665592
↑ Wang X, Khaleque MA, Zhao MJ, Zhong R, Gaestel M, Calderwood SK. Phosphorylation of HSF1 by MAPK-activated protein kinase 2 on serine 121, inhibits transcriptional activity and promotes HSP90 binding. J Biol Chem. 2006 Jan 13;281(2):782-91. Epub 2005 Nov 8. PMID:16278218 doi:M505822200

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OCA

[1] Holmberg CI, Hietakangas V, Mikhailov A, Rantanen JO, Kallio M, Meinander A, Hellman J, Morrice N, MacKintosh C, Morimoto RI, Eriksson JE, Sistonen L. Phosphorylation of serine 230 promotes inducible transcriptional activity of heat shock factor 1. EMBO J. 2001 Jul 16;20(14):3800-10. PMID:11447121 doi:https://dx.doi.org/10.1093/emboj/20.14.3800

[2] Knauf U, Newton EM, Kyriakis J, Kingston RE. Repression of human heat shock factor 1 activity at control temperature by phosphorylation. Genes Dev. 1996 Nov 1;10(21):2782-93. PMID:8946918

[3] Kline MP, Morimoto RI. Repression of the heat shock factor 1 transcriptional activation domain is modulated by constitutive phosphorylation. Mol Cell Biol. 1997 Apr;17(4):2107-15. PMID:9121459

[4] Xia W, Guo Y, Vilaboa N, Zuo J, Voellmy R. Transcriptional activation of heat shock factor HSF1 probed by phosphopeptide analysis of factor 32P-labeled in vivo. J Biol Chem. 1998 Apr 10;273(15):8749-55. PMID:9535852

[5] Guo Y, Guettouche T, Fenna M, Boellmann F, Pratt WB, Toft DO, Smith DF, Voellmy R. Evidence for a mechanism of repression of heat shock factor 1 transcriptional activity by a multichaperone complex. J Biol Chem. 2001 Dec 7;276(49):45791-9. Epub 2001 Oct 2. PMID:11583998 doi:https://dx.doi.org/10.1074/jbc.M105931200

[6] Soncin F, Zhang X, Chu B, Wang X, Asea A, Ann Stevenson M, Sacks DB, Calderwood SK. Transcriptional activity and DNA binding of heat shock factor-1 involve phosphorylation on threonine 142 by CK2. Biochem Biophys Res Commun. 2003 Apr 4;303(2):700-6. PMID:12659875

[7] Hietakangas V, Ahlskog JK, Jakobsson AM, Hellesuo M, Sahlberg NM, Holmberg CI, Mikhailov A, Palvimo JJ, Pirkkala L, Sistonen L. Phosphorylation of serine 303 is a prerequisite for the stress-inducible SUMO modification of heat shock factor 1. Mol Cell Biol. 2003 Apr;23(8):2953-68. PMID:12665592

[8] Wang X, Khaleque MA, Zhao MJ, Zhong R, Gaestel M, Calderwood SK. Phosphorylation of HSF1 by MAPK-activated protein kinase 2 on serine 121, inhibits transcriptional activity and promotes HSP90 binding. J Biol Chem. 2006 Jan 13;281(2):782-91. Epub 2005 Nov 8. PMID:16278218 doi:M505822200

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+==Crystal structure of human Hsf1 with Satellite III repeat DNA==
+<StructureSection load='5d5v' size='340' side='right' caption='[[5d5v]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
-The entry 5d5v is ON HOLD  until Paper Publication
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5d5v]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5D5V FirstGlance]. <br>
-Authors: Bracher, A.
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5d5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d5v OCA], [http://pdbe.org/5d5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5d5v RCSB], [http://www.ebi.ac.uk/pdbsum/5d5v PDBsum]</span></td></tr>
-Description:
+</table>
-[[Category: Unreleased Structures]]
+== Function ==
+[[http://www.uniprot.org/uniprot/HSF1_HUMAN HSF1_HUMAN]] DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.<ref>PMID:11447121</ref> <ref>PMID:8946918</ref> <ref>PMID:9121459</ref> <ref>PMID:9535852</ref> <ref>PMID:11583998</ref> <ref>PMID:12659875</ref> <ref>PMID:12665592</ref> <ref>PMID:16278218</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Bracher, A]]
+[[Category: Double helix]]
+[[Category: Helix-turn-helix]]
+[[Category: Protein-dna complex]]
+[[Category: Transcription-dna complex]]

5d5v: Difference between revisions

Revision as of 19:38, 30 December 2015

Crystal structure of human Hsf1 with Satellite III repeat DNA

Structural highlights

Function

References

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