1fhy: Difference between revisions
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|PDB= 1fhy |SIZE=350|CAPTION= <scene name='initialview01'>1fhy</scene>, resolution 2.20Å | |PDB= 1fhy |SIZE=350|CAPTION= <scene name='initialview01'>1fhy</scene>, resolution 2.20Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=PSO:4'-HYDROXYMETHYL-4,5',8-TRIMETHYLPSORALEN'>PSO</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fhy OCA], [http://www.ebi.ac.uk/pdbsum/1fhy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1fhy RCSB]</span> | |||
}} | }} | ||
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[[Category: Ho, P S.]] | [[Category: Ho, P S.]] | ||
[[Category: Mooers, B H.M.]] | [[Category: Mooers, B H.M.]] | ||
[[Category: 4-way junction]] | [[Category: 4-way junction]] | ||
[[Category: cross-linked dna]] | [[Category: cross-linked dna]] | ||
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[[Category: psoralen]] | [[Category: psoralen]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:23:15 2008'' | ||
Revision as of 17:23, 30 March 2008
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| 1fhy, resolution 2.20Å | |||||||||||||
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| Ligands: | CA, DA, DC, DG, DT, PSO | ||||||||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
PSORALEN CROSS-LINKED D(CCGCTAGCGG) FORMS HOLLIDAY JUNCTION
Overview
The single-crystal structures are presented for two DNA sequences with the thymine bases covalently cross-linked across the complementary strands by 4'-hydroxymethyl-4,5',8-trimethylpsoralen (HMT). The HMT-adduct of d(CCGCTAGCGG) forms a psoralen-induced Holliday junction, showing for the first time the effect of this important class of chemotheraputics on the structure of the recombination intermediate. In contrast, HMT-d(CCGGTACCGG) forms a sequence-dependent junction. In both structures, the DNA duplex is highly distorted at the thymine base linked to the six-member pyrone ring of the drug. The psoralen cross-link defines the intramolecular interactions of the drug-induced junction, while the sequence-dependent structure is nearly identical to the native Holliday junction of d(CCGGTACCGG) alone. The two structures contrast the effects of drug- and sequence-dependent interactions on the structure of a Holliday junction, suggesting a role for psoralen in the mechanism to initiate repair of psoralen-lesions in mammalian DNA.
About this Structure
1FHY is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
The crystal structures of psoralen cross-linked DNAs: drug-dependent formation of Holliday junctions., Eichman BF, Mooers BH, Alberti M, Hearst JE, Ho PS, J Mol Biol. 2001 Apr 20;308(1):15-26. PMID:11302703
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