1f8m: Difference between revisions
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|GENE= | |GENE= | ||
|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG2224 AceA]</span> | |DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG2224 AceA]</span> | ||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f8m OCA], [http://www.ebi.ac.uk/pdbsum/1f8m PDBsum | |RELATEDENTRY=[[1f61|1F61]], [[1f8i|1F8I]] | ||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f8m OCA], [http://www.ebi.ac.uk/pdbsum/1f8m PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1f8m RCSB]</span> | |||
}} | }} | ||
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[[Category: tbsgc]] | [[Category: tbsgc]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:18:06 2008'' | ||
Revision as of 17:18, 30 March 2008
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| 1f8m, resolution 1.80Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | MG, PYR | ||||||||||||
| Activity: | Isocitrate lyase, with EC number 4.1.3.1 | ||||||||||||
| Domains: | AceA | ||||||||||||
| Related: | 1F61, 1F8I
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
CRYSTAL STRUCTURE OF 3-BROMOPYRUVATE MODIFIED ISOCITRATE LYASE (ICL) FROM MYCOBACTERIUM TUBERCULOSIS
Overview
Isocitrate lyase (ICL) plays a pivotal role in the persistence of Mycobacterium tuberculosis in mice by sustaining intracellular infection in inflammatory macrophages. The enzyme allows net carbon gain by diverting acetyl-CoA from beta-oxidation of fatty acids into the glyoxylate shunt pathway. Given its potential as a drug target against persistent infections, we solved its structure without ligand and in complex with two inhibitors. Covalent modification of an active site residue, Cys 191, by the inhibitor 3-bromopyruvate traps the enzyme in a catalytic conformation with the active site completely inaccessible to solvent. The structure of a C191S mutant of the enzyme with the inhibitor 3-nitropropionate provides further insight into the reaction mechanism.
About this Structure
1F8M is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.
Reference
Structure of isocitrate lyase, a persistence factor of Mycobacterium tuberculosis., Sharma V, Sharma S, Hoener zu Bentrup K, McKinney JD, Russell DG, Jacobs WR Jr, Sacchettini JC, Nat Struct Biol. 2000 Aug;7(8):663-8. PMID:10932251
Page seeded by OCA on Sun Mar 30 20:18:06 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Isocitrate lyase
- Mycobacterium tuberculosis
- Single protein
- Bentrup, K Hoener zu.
- Jr., W R.Jacobs.
- McKinney, J D.
- Russell, D G.
- Sacchettini, J C.
- Sharma, S.
- Sharma, V.
- TBSGC, TB Structural Genomics Consortium.
- Alpha-beta barrel
- Bromopyuvate modification
- Closed conformation
- Helix-swapping
- Protein structure initiative
- Psi
- Structural genomic
- Tb structural genomics consortium
- Tbsgc
