2rc3: Difference between revisions
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|GENE= NE2398 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=228410 Nitrosomonas europaea ATCC 19718]) | |GENE= NE2398 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=228410 Nitrosomonas europaea ATCC 19718]) | ||
|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd04623 CBS_pair_10], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd04642 CBS_pair_29]</span> | |DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd04623 CBS_pair_10], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd04642 CBS_pair_29]</span> | ||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rc3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rc3 OCA], [http://www.ebi.ac.uk/pdbsum/2rc3 PDBsum | |RELATEDENTRY= | ||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rc3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rc3 OCA], [http://www.ebi.ac.uk/pdbsum/2rc3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2rc3 RCSB]</span> | |||
}} | }} | ||
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[[Category: unknown function]] | [[Category: unknown function]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:58:49 2008'' | ||
Revision as of 01:58, 31 March 2008
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| 2rc3, resolution 1.60Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | BR, NAD | ||||||||||||
| Gene: | NE2398 (Nitrosomonas europaea ATCC 19718) | ||||||||||||
| Domains: | CBS_pair_10, CBS_pair_29 | ||||||||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of CBS domain, NE2398
Overview
We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.
About this Structure
2RC3 is a Single protein structure of sequence from Nitrosomonas europaea atcc 19718. Full crystallographic information is available from OCA.
Reference
In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4. PMID:17982461
Page seeded by OCA on Mon Mar 31 04:58:49 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Nitrosomonas europaea atcc 19718
- Single protein
- Dong, A.
- Edwards, A M.
- Joachimiak, A.
- Korniyenko, Y.
- MCSG, Midwest Center for Structural Genomics.
- Savchenko, A.
- Walker, J R.
- Xu, X.
- Yakunin, A.
- Zheng, H.
- Br
- Cbs domain
- In situ proteolysis
- Mcsg
- Midwest center for structural genomic
- Protein structure initiative
- Psi-2
- Structural genomic
- Unknown function
