4z8v: Difference between revisions

Revision as of 16:05, 15 November 2017

CRYSTAL STRUCTURE OF AVRRXO1-ORF1:-ORF2 COMPLEX, NATIVE.

Structural highlights

4z8v is a 2 chain structure with sequence from Xanthomonas campestris pv. oryzicola. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	PO4
Related:	4z8q, 4z8t, 4z8u
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Xanthomonas oryzae pv. oryzicola (Xoc) causes bacterial leaf streak (BLS) disease on rice plants. Xoc delivers a type III effector AvrRxo1-ORF1 into rice plant cells that can be recognized by disease resistance (R) protein Rxo1, and triggers resistance to BLS disease. However, the mechanism and virulence role of AvrRxo1 is not known. In the genome of Xoc, AvrRxo1-ORF1 is adjacent to another gene AvrRxo1-ORF2, which was predicted to encode a molecular chaperone of AvrRxo1-ORF1. We report the co-purification and crystallization of the AvrRxo1-ORF1:AvrRxo1-ORF2 tetramer complex at 1.64 A resolution. AvrRxo1-ORF1 has a T4 polynucleotide kinase domain, and expression of AvrRxo1-ORF1 suppresses bacterial growth in a manner dependent on the kinase motif. Although AvrRxo1-ORF2 binds AvrRxo1-ORF1, it is structurally different from typical effector-binding chaperones, in that it has a distinct fold containing a novel kinase-binding domain. AvrRxo1-ORF2 functions to suppress the bacteriostatic activity of AvrRxo1-ORF1 in bacterial cells.

Crystal Structure of Xanthomonas AvrRxo1-ORF1, a Type III Effector with a Polynucleotide Kinase Domain, and Its Interactor AvrRxo1-ORF2.,Han Q, Zhou C, Wu S, Liu Y, Triplett L, Miao J, Tokuhisa J, Deblais L, Robinson H, Leach JE, Li J, Zhao B Structure. 2015 Oct 6;23(10):1900-9. doi: 10.1016/j.str.2015.06.030. Epub 2015, Sep 3. PMID:26344722^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Han Q, Zhou C, Wu S, Liu Y, Triplett L, Miao J, Tokuhisa J, Deblais L, Robinson H, Leach JE, Li J, Zhao B. Crystal Structure of Xanthomonas AvrRxo1-ORF1, a Type III Effector with a Polynucleotide Kinase Domain, and Its Interactor AvrRxo1-ORF2. Structure. 2015 Oct 6;23(10):1900-9. doi: 10.1016/j.str.2015.06.030. Epub 2015, Sep 3. PMID:26344722 doi:https://dx.doi.org/10.1016/j.str.2015.06.030

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OCA

[1] Han Q, Zhou C, Wu S, Liu Y, Triplett L, Miao J, Tokuhisa J, Deblais L, Robinson H, Leach JE, Li J, Zhao B. Crystal Structure of Xanthomonas AvrRxo1-ORF1, a Type III Effector with a Polynucleotide Kinase Domain, and Its Interactor AvrRxo1-ORF2. Structure. 2015 Oct 6;23(10):1900-9. doi: 10.1016/j.str.2015.06.030. Epub 2015, Sep 3. PMID:26344722 doi:https://dx.doi.org/10.1016/j.str.2015.06.030

[1]

@@ Line 1: / Line 1: @@
 ==CRYSTAL STRUCTURE OF AVRRXO1-ORF1:-ORF2 COMPLEX, NATIVE.==
 <StructureSection load='4z8v' size='340' side='right' caption='[[4z8v]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4z8v]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z8V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z8V FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4z8v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Xanthomonas_campestris_pv._oryzicola Xanthomonas campestris pv. oryzicola]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z8V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z8V FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4z8q|4z8q]], [[4z8t|4z8t]], [[4z8u|4z8u]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z8v OCA], [http://pdbe.org/4z8v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z8v RCSB], [http://www.ebi.ac.uk/pdbsum/4z8v PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z8v OCA], [http://pdbe.org/4z8v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z8v RCSB], [http://www.ebi.ac.uk/pdbsum/4z8v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4z8v ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 20: / Line 21: @@
 __TOC__
 </StructureSection>
+[[Category: Xanthomonas campestris pv. oryzicola]]
 [[Category: Cheng, Q]]
 [[Category: Deblais, L]]

4z8v: Difference between revisions

Revision as of 16:05, 15 November 2017

CRYSTAL STRUCTURE OF AVRRXO1-ORF1:-ORF2 COMPLEX, NATIVE.

Structural highlights

Publication Abstract from PubMed

References

Contents

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