Barnase

<StructureSection load='1brn' size='450' side='right' scene= caption='Barnase complex with tetranucleotide (PDB code 1brn)'>

Function

Barnase (BA, BActerial RiboNucleASE) is a bacterial ribonuclease synthesized and secreted by the bacterium Bacillus amyloliquefaciens. The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA.

Relevance

BA structure was used extensively for studying protein folding.

Structural highlights

BA hydrolyzes RNA at GpN sites where the positively charge Lys and Arg coordinate the RNA phosphate group.

3D Structures of Barnase

Updated on 09-November-2015

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• Barnase
  • 3kch, 2f4y – BA cross-linked
    
  • 2f56 - BA cross-linked + urea
    
  • 2f5w - BA cross-linked + thiourea
    
  • 2f5m - BA cross-linked + Bromo-ethanol
    
  • 1fw7, 1bnr – BA - NMR
    
  • 2kf3 – BA (mutant) – NMR
    
  • 2kf4, 1b2x, 1a2p, 1bni, 1bnj – BA
    
  • 1yvs – BA domain swapped
    
  • 1b20, 1b21, 1b2z, 1bne, 1bnf, 1bng, 1brh, 1bri, 1brj, 1brk, 1bns, 1brg, 1bsa, 1bsb, 1bsc, 1bsd, 1bse, 1ban, 1bao – BA (mutant)
    
  • 2kf5, 2kf6, 1brn, 1rnb – BA + polynucleotide
• Barnase + BST
  • 3da7 - BA (mutant) + BST
    
  • 2za4, 1brs, 1bgs – BA + BST
    
  • 1x1u, 1x1w, 1x1x, 1x1y, 1b27, 1b2u – BA + BST (mutant)
    
  • 1b3s, 1b2s - BA (mutant) + BST (mutant)
    
  • 2c4b – BA/MCOEETI (mutant)

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