4v17: Difference between revisions
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''' | ==Structure of a novel carbohydrate binding module from glycoside hydrolase family 5 glucanase from Ruminococcus flavefaciens FD-1== | ||
<StructureSection load='4v17' size='340' side='right' caption='[[4v17]], [[Resolution|resolution]] 2.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4v17]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V17 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4V17 FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4v18|4v18]], [[4v1b|4v1b]], [[4v1i|4v1i]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4v17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v17 OCA], [http://pdbe.org/4v17 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4v17 RCSB], [http://www.ebi.ac.uk/pdbsum/4v17 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Anaerobic bacteria organize carbohydrate-active enzymes into a multi-component complex, the cellulosome, which degrades cellulose and hemicellulose highly efficiently. Genome sequencing of Ruminococcus flavefaciens FD-1 offers extensive information on the range and diversity of the enzymatic and structural components of the cellulosome. The R. flavefaciens FD-1 genome encodes over 200 dockerin-containing proteins, most of which are of unknown function. One of these modular proteins comprises a glycoside hydrolase family 5 catalytic module (GH5) linked to an unclassified carbohydrate-binding module (CBM-Rf1) and a dockerin. The novel CBM-Rf1 has been purified and crystallized. The crystals belonged to the trigonal space group R32:H. The CBM-Rf1 structure was determined by a multiple-wavelength anomalous dispersion experiment using AutoSol from the PHENIX suite using both selenomethionyl-derivative and native data to resolutions of 2.28 and 2.0 A, respectively. | |||
Expression, purification and crystallization of a novel carbohydrate-binding module from the Ruminococcus flavefaciens cellulosome.,Venditto I, Centeno MS, Ferreira LM, Fontes CM, Najmudin S Acta Crystallogr F Struct Biol Commun. 2014 Dec 1;70(Pt 12):1653-6. doi:, 10.1107/S2053230X14024248. Epub 2014 Nov 14. PMID:25484220<ref>PMID:25484220</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4v17" style="background-color:#fffaf0;"></div> | |||
[[Category: | == References == | ||
[[Category: Ferreira, L.M.A]] | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Centeno, M S.J]] | |||
[[Category: Ferreira, L M.A]] | |||
[[Category: Fontes, C M.G A]] | |||
[[Category: Najmudin, S]] | [[Category: Najmudin, S]] | ||
[[Category: Venditto, I]] | [[Category: Venditto, I]] | ||
[[Category: | [[Category: Sugar binding protein]] | ||
Revision as of 16:54, 20 January 2016
Structure of a novel carbohydrate binding module from glycoside hydrolase family 5 glucanase from Ruminococcus flavefaciens FD-1
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