4xsu: Difference between revisions

Line 1:

'''~~Unreleased~~ structure'''

==Crystal structure of Anabaena Alr3699/HepE in complex with UDP and glucose==

== Structural highlights ==

<table><tr><td colspan='2'>[[4xsu]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XSU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XSU FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4xso|4xso]], [[4xsp|4xsp]], [[4xsr|4xsr]]</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xsu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xsu OCA], [http://pdbe.org/4xsu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xsu RCSB], [http://www.ebi.ac.uk/pdbsum/4xsu PDBsum]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

Formation of the heterocyst envelope polysaccharide (HEP) is a key process for cyanobacterial heterocyst differentiation. The maturation of HEP in Anabaena sp. strain PCC 7120 is controlled by a gene cluster termed HEP island in addition to an operon alr3698-alr3699, which encodes two putative proteins termed Alr3698/HepD and Alr3699/HepE. Here we report the crystal structures of HepE in the apo-form and three complex forms that bind to UDP-glucose (UDPG), UDP&glucose and UDP, respectively. The overall structure of HepE displays a typical GT-B fold of glycosyltransferases, comprising two separate beta/alpha/beta Rossmann-fold domains that form an inter-domain substrate-binding crevice. Structural analyses combined with enzymatic assays indicate that HepE is a glucosyltransferase using UDPG as a sugar donor. Further site-directed mutageneses enable us to assign the key residues that stabilize the sugar donor and putative acceptor. Based on the comparative structural analyses, we propose a putative catalytic cycle of HepE, which undergoes "open-closed-open" conformational changes upon binding to the substrates and release of products. These findings provide structural and catalytic insights into the first enzyme involved in the HEP biosynthesis pathway.

~~The entry 4xsu is ON HOLD until Paper Publication~~

Structural and enzymatic analyses of a glucosyltransferase Alr3699/HepE involved in Anabaena heterocyst envelop polysaccharide biosynthesis.,Wang XP, Jiang YL, Dai YN, Cheng W, Chen Y, Zhou CZ Glycobiology. 2015 Dec 21. pii: cwv167. PMID:26692049<ref>PMID:26692049</ref>

~~Authors: Wang~~, X.P.~~, Dai, Y.N., Jiang, Y.L., Cheng, W., Chen, Y.X., Zhou, C.Z~~.

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

~~Description~~: ~~Crystal structure of Anabaena Alr3699 in complex with UDP and glucose~~

~~[[Category: Unreleased Structures]]~~

== References ==

~~[[Category: Chen, Y.X]]~~

~~[[Category: Dai, Y.N]]~~

__TOC__

~~[[Category: Wang, X.P]]~~

</StructureSection>

[[Category: ~~Jiang~~, Y.L]]

[[Category: Chen, Y X]]

[[Category: Cheng, W]]

[[Category: Zhou, C.Z]]

[[Category: Dai, Y N]]

[[Category: Jiang, Y L]]

[[Category: Wang, X P]]

[[Category: Zhou, C Z]]

[[Category: Glycosyltransferase]]

[[Category: Gt-b fold]]

[[Category: Transferase]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+==Crystal structure of Anabaena Alr3699/HepE in complex with UDP and glucose==
+<StructureSection load='4xsu' size='340' side='right' caption='[[4xsu]], [[Resolution|resolution]] 2.48&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4xsu]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XSU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XSU FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4xso|4xso]], [[4xsp|4xsp]], [[4xsr|4xsr]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xsu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xsu OCA], [http://pdbe.org/4xsu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xsu RCSB], [http://www.ebi.ac.uk/pdbsum/4xsu PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Formation of the heterocyst envelope polysaccharide (HEP) is a key process for cyanobacterial heterocyst differentiation. The maturation of HEP in Anabaena sp. strain PCC 7120 is controlled by a gene cluster termed HEP island in addition to an operon alr3698-alr3699, which encodes two putative proteins termed Alr3698/HepD and Alr3699/HepE. Here we report the crystal structures of HepE in the apo-form and three complex forms that bind to UDP-glucose (UDPG), UDP&amp;glucose and UDP, respectively. The overall structure of HepE displays a typical GT-B fold of glycosyltransferases, comprising two separate beta/alpha/beta Rossmann-fold domains that form an inter-domain substrate-binding crevice. Structural analyses combined with enzymatic assays indicate that HepE is a glucosyltransferase using UDPG as a sugar donor. Further site-directed mutageneses enable us to assign the key residues that stabilize the sugar donor and putative acceptor. Based on the comparative structural analyses, we propose a putative catalytic cycle of HepE, which undergoes "open-closed-open" conformational changes upon binding to the substrates and release of products. These findings provide structural and catalytic insights into the first enzyme involved in the HEP biosynthesis pathway.
-The entry 4xsu is ON HOLD  until Paper Publication
+Structural and enzymatic analyses of a glucosyltransferase Alr3699/HepE involved in Anabaena heterocyst envelop polysaccharide biosynthesis.,Wang XP, Jiang YL, Dai YN, Cheng W, Chen Y, Zhou CZ Glycobiology. 2015 Dec 21. pii: cwv167. PMID:26692049<ref>PMID:26692049</ref>
-Authors: Wang, X.P., Dai, Y.N., Jiang, Y.L., Cheng, W., Chen, Y.X., Zhou, C.Z.
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-Description: Crystal structure of Anabaena Alr3699 in complex with UDP and glucose
+<div class="pdbe-citations 4xsu" style="background-color:#fffaf0;"></div>
-[[Category: Unreleased Structures]]
+== References ==
-[[Category: Chen, Y.X]]
+<references/>
-[[Category: Dai, Y.N]]
+__TOC__
-[[Category: Wang, X.P]]
+</StructureSection>
-[[Category: Jiang, Y.L]]
+[[Category: Chen, Y X]]
 [[Category: Cheng, W]]
-[[Category: Zhou, C.Z]]
+[[Category: Dai, Y N]]
+[[Category: Jiang, Y L]]
+[[Category: Wang, X P]]
+[[Category: Zhou, C Z]]
+[[Category: Glycosyltransferase]]
+[[Category: Gt-b fold]]
+[[Category: Transferase]]