5ehk: Difference between revisions
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==Crystal structure of tRNA dependent lantibiotic dehydratase MibB from Microbispora sp. 107891== | |||
<StructureSection load='5ehk' size='340' side='right' caption='[[5ehk]], [[Resolution|resolution]] 2.71Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5ehk]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EHK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5EHK FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ehk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ehk OCA], [http://pdbe.org/5ehk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ehk RCSB], [http://www.ebi.ac.uk/pdbsum/5ehk PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Class I lantibiotic dehydratases dehydrate selected Ser/Thr residues of a precursor peptide. Recent studies demonstrated the requirement of glutamyl-tRNAGlu for Ser/Thr activation by one of these enzymes (NisB) from the Firmicute Lactococcus lactis. However, the generality of glutamyl-tRNAGlu usage and the tRNA specificity of lantibiotic dehydratases have not been established. Here we report the 2.7-A resolution crystal structure, along with the glutamyl-tRNAGlu utilization of MibB, a lantibiotic dehydratase from the Actinobacterium Microbispora sp. 107891 involved in the biosynthesis of the clinical candidate NAI-107. Biochemical assays revealed nucleotides A73 and U72 within the tRNAGlu acceptor stem to be important for MibB glutamyl-tRNAGlu usage. Using this knowledge, an expression system for the production of NAI-107 analogs in Escherichia coli was developed, overcoming the inability of MibB to utilize E. coli tRNAGlu. Our work provides evidence for a common tRNAGlu-dependent dehydration mechanism, paving the way for the characterization of lantibiotics from various phyla. | |||
Structure and tRNA Specificity of MibB, a Lantibiotic Dehydratase from Actinobacteria Involved in NAI-107 Biosynthesis.,Ortega MA, Hao Y, Walker MC, Donadio S, Sosio M, Nair SK, van der Donk WA Cell Chem Biol. 2016 Feb 8. pii: S2451-9456(16)30001-0. doi:, 10.1016/j.chembiol.2015.11.017. PMID:26877024<ref>PMID:26877024</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 5ehk" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Hao, Y]] | [[Category: Hao, Y]] | ||
[[Category: Nair, S K]] | |||
[[Category: Hydrolase]] | |||
[[Category: Lantibiotic dehydratase]] | |||
[[Category: Microbispora sp. 107891]] | |||
[[Category: Nai-107]] | |||
[[Category: Trna dependent]] | |||
Revision as of 15:09, 2 March 2016
Crystal structure of tRNA dependent lantibiotic dehydratase MibB from Microbispora sp. 107891
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