5ep5

The crystal structure of the hypothetical protein SAV0944 mutant (Glu47Ala) from Staphylococcus aureus.

spinqualitylabelsall models 5ep5, resolution 2.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5ep5 is a 4 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 4m20, 5eo5 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum Publication Abstract from PubMed Staphylococcus aureus is the causative agent of many diseases, including meningitis, bacteraemia, pneumonia, food poisoning and toxic shock syndrome. Structural characterization of the PaaI-like thioesterase SAV0944 (SaPaaI) from S. aureus subsp. aureus Mu50 will aid in understanding its potential as a new therapeutic target by knowledge of its molecular details and cellular functions. Here, the recombinant expression, purification and crystallization of SaPaaI thioesterase from S. aureus are reported. This protein initially crystallized with the ligand coenzyme A using the hanging-drop vapour-diffusion technique with condition No. 40 of Crystal Screen from Hampton Research at 296 K. Optimal final conditions consisting of 24% PEG 4000, 100 mM sodium citrate pH 6.5, 12% 2-propanol gave single diffraction-quality crystals. These crystals diffracted to beyond 2 A resolution at the Australian Synchrotron and belonged to space group P12(1)1, with unit-cell parameters a = 44.05, b = 89.05, c = 60.74 A, beta = 100.5 degrees . Initial structure determination and refinement gave an R factor and R(free) of 17.3 and 22.0%, respectively, confirming a positive solution in obtaining phases using molecular replacement. Expression, purification, crystallization and preliminary X-ray analysis of the PaaI-like thioesterase SAV0944 from Staphylococcus aureus.,Khandokar YB, Roman N, Smith KM, Srivastava P, Forwood JK Acta Crystallogr F Struct Biol Commun. 2014 Feb;70(Pt 2):244-7. doi:, 10.1107/S2053230X14000338. Epub 2014 Jan 22. PMID:24637766 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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