User:Michael Adams/Sandbox 1: Difference between revisions
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A phosphokinase used to store energy in the form of Argininephosphate. | A phosphokinase used to store energy in the form of Argininephosphate. | ||
== Isolation == | == Isolation == | ||
In Strong and Ellington’s 1994 experiment, arginine kinase (AK) was isolated from Limulus polyphemus, the Atlantic horseshoe crab, a marine chelicerate arthropod. They isolated the gene for AK, sequenced the DNA and identified it to be 1071 nucleotides. The 1071 nucleotides produce a 357 amino acid protein and AK extracted from other organisms show similarity to this protein. AK serves a similar function to that of creatine kinase, in vertebrates <ref>Strong, S., & Ellington, W. (1994). Isolation and sequence analysis of the gene for | In Strong and Ellington’s 1994 experiment, arginine kinase (AK) was isolated from <i>Limulus polyphemus</i>, the Atlantic horseshoe crab, a marine chelicerate arthropod. They isolated the gene for AK, sequenced the DNA and identified it to be 1071 nucleotides. The 1071 nucleotides produce a 357 amino acid protein and AK extracted from other organisms show similarity to this protein. AK serves a similar function to that of creatine kinase, in vertebrates <ref>Strong, S., & Ellington, W. (1994). Isolation and sequence analysis of the gene for | ||
arginine kinase from the chelicerate arthropod, Limulus polyphemus: Insights into | arginine kinase from the chelicerate arthropod, Limulus polyphemus: Insights into | ||
catalytically important residues. Biochimica Et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 197-200.</ref>. The enzyme creatine kinase maintains energy homeostasis by producing ATP in high energy requiring cells such as skeletal and cardiac muscle and neurons <ref name=wallimann1973>Wallimann, Theo, and Hans M. Eppenberger. "Properties of Arginine Kinase from | catalytically important residues. Biochimica Et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 197-200.</ref>. The enzyme creatine kinase maintains energy homeostasis by producing ATP in high energy requiring cells such as skeletal and cardiac muscle and neurons <ref name=wallimann1973>Wallimann, Theo, and Hans M. Eppenberger. "Properties of Arginine Kinase from | ||
