4win: Difference between revisions

Revision as of 09:09, 23 May 2019

Crystal structure of the GATase domain from Plasmodium falciparum GMP synthetase

Structural highlights

4win is a 2 chain structure with sequence from Plaf7. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	NO3
Related:	4wim, 4wio
Gene:	PF10_0123 (PLAF7)
Activity:	GMP synthase (glutamine-hydrolyzing), with EC number 6.3.5.2
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

GMP synthetase (GMPS), a key enzyme in the purine biosynthetic pathway performs catalysis through a coordinated process across two catalytic pockets for which the mechanism remains unclear. Crystal structures of Plasmodium falciparum GMPS in conjunction with mutational and enzyme kinetic studies reported here provide evidence that an 85 degrees rotation of the GATase domain is required for ammonia channelling and thus for the catalytic activity of this two-domain enzyme. We suggest that conformational changes in helix 371-375 holding catalytic residues and in loop 376-401 along the rotation trajectory trigger the different steps of catalysis, and establish the central role of Glu374 in allostery and inter-domain crosstalk. These studies reveal the mechanism of domain rotation and inter-domain communication, providing a molecular framework for the function of all single polypeptide GMPSs and form a solid basis for rational drug design targeting this therapeutically important enzyme.

Active site coupling in Plasmodium falciparum GMP synthetase is triggered by domain rotation.,Ballut L, Violot S, Shivakumaraswamy S, Thota LP, Sathya M, Kunala J, Dijkstra BW, Terreux R, Haser R, Balaram H, Aghajari N Nat Commun. 2015 Nov 23;6:8930. doi: 10.1038/ncomms9930. PMID:26592566^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ballut L, Violot S, Shivakumaraswamy S, Thota LP, Sathya M, Kunala J, Dijkstra BW, Terreux R, Haser R, Balaram H, Aghajari N. Active site coupling in Plasmodium falciparum GMP synthetase is triggered by domain rotation. Nat Commun. 2015 Nov 23;6:8930. doi: 10.1038/ncomms9930. PMID:26592566 doi:https://dx.doi.org/10.1038/ncomms9930

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OCA

[1] Ballut L, Violot S, Shivakumaraswamy S, Thota LP, Sathya M, Kunala J, Dijkstra BW, Terreux R, Haser R, Balaram H, Aghajari N. Active site coupling in Plasmodium falciparum GMP synthetase is triggered by domain rotation. Nat Commun. 2015 Nov 23;6:8930. doi: 10.1038/ncomms9930. PMID:26592566 doi:https://dx.doi.org/10.1038/ncomms9930

[1]

@@ Line 1: / Line 1: @@
 ==Crystal structure of the GATase domain from Plasmodium falciparum GMP synthetase==
-<StructureSection load='4win' size='340' side='right' caption='[[4win]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
+<StructureSection load='4win' size='340' side='right'caption='[[4win]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4win]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WIN FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4win]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Plaf7 Plaf7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WIN FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4wim|4wim]], [[4wio|4wio]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PF10_0123 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36329 PLAF7])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/GMP_synthase_(glutamine-hydrolyzing) GMP synthase (glutamine-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.5.2 6.3.5.2] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4win FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4win OCA], [http://pdbe.org/4win PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4win RCSB], [http://www.ebi.ac.uk/pdbsum/4win PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4win FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4win OCA], [http://pdbe.org/4win PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4win RCSB], [http://www.ebi.ac.uk/pdbsum/4win PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4win ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 21: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
+[[Category: Plaf7]]
 [[Category: Aghajari, N]]
 [[Category: Ballut, L]]

4win: Difference between revisions

Revision as of 09:09, 23 May 2019

Crystal structure of the GATase domain from Plasmodium falciparum GMP synthetase

Structural highlights

Publication Abstract from PubMed

References

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