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{{STRUCTURE_1b4c| PDB=1b4c | SCENE= }} | |||
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'''SOLUTION STRUCTURE OF RAT APO-S100B USING DIPOLAR COUPLINGS''' | '''SOLUTION STRUCTURE OF RAT APO-S100B USING DIPOLAR COUPLINGS''' | ||
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[[Category: Tjandra, N.]] | [[Category: Tjandra, N.]] | ||
[[Category: Weber, D J.]] | [[Category: Weber, D J.]] | ||
[[Category: | [[Category: Calcium- binding protein]] | ||
[[Category: | [[Category: Dipolar coupling]] | ||
[[Category: | [[Category: Ef-hand]] | ||
[[Category: | [[Category: Four-helix bundle]] | ||
[[Category: | [[Category: Nmr]] | ||
[[Category: | [[Category: S100 protein]] | ||
[[Category: | [[Category: S100b]] | ||
[[Category: | [[Category: S100beta]] | ||
[[Category: | [[Category: Solution structure]] | ||
[[Category: | [[Category: Three-dimensional structure]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:03:05 2008'' | |||
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Revision as of 08:03, 2 May 2008
SOLUTION STRUCTURE OF RAT APO-S100B USING DIPOLAR COUPLINGS
Overview
The relative orientations of adjacent structural elements without many well-defined NOE contacts between them are typically poorly defined in NMR structures. For apo-S100B(betabeta) and the structurally homologous protein calcyclin, the solution structures determined by conventional NMR exhibited considerable differences and made it impossible to draw unambiguous conclusions regarding the Ca2+-induced conformational change required for target protein binding. The structure of rat apo-S100B(betabeta) was recalculated using a large number of constraints derived from dipolar couplings that were measured in a dilute liquid crystalline phase. The dipolar couplings orient bond vectors relative to a single-axis system, and thereby remove much of the uncertainty in NOE-based structures. The structure of apo-S100B(betabeta) indicates a minimal change in the first, pseudo-EF-hand Ca2+ binding site, but a large reorientation of helix 3 in the second, classical EF-hand upon Ca2+ binding.
About this Structure
1B4C is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
The use of dipolar couplings for determining the solution structure of rat apo-S100B(betabeta)., Drohat AC, Tjandra N, Baldisseri DM, Weber DJ, Protein Sci. 1999 Apr;8(4):800-9. PMID:10211826 Page seeded by OCA on Fri May 2 11:03:05 2008
