Clathrin JMU: Difference between revisions

Line 12:

===Lattice===

Individual clathrin molecules can assemble into cage-like structures. When individual clathrin molecules come together, a lattice is formed where each lattice point is associated with the center of a triskelion (from the Greek word meaning “a three legged structure”) (Figure 1).<ref name="harrison" /> According to Crowther, R.A., clathrin vesicles are constructed from twelve pentagonal units plus a number of hexagonal units, with the number of hexagons increasing for larger clathrin coats.<ref name="crowther">PMID: 7328122 </ref> The term “cage” can be used to refer to empty shells composed only of clathrin while the term “coat” refers to clathrin in addition to associated proteins and vesicles.<ref name="crowther" /> Clathrin lattices are formed under optimal conditions of physiological pH (6.0-6.5) and in the presence of Calcium or Magnesium ions.<ref name="crowther" /> Various packing models have been investigated as to how the individual triskelion legs assemble into clathrin cages.<ref name="crowther" /> As mentioned above, the center of the triskelion legs occurs at each vertex of the cage, meaning pentagons, heptagons, and hexagons can be formed.<ref name="crowther" /> In the individual triskelion legs, the bend in the leg is 160 Angstroms from the vertex, giving rise to two models of packing: simple side-by-side packing or a cross-over type of packing (Figure 2).<ref name="crowther" /> Cross-over packing occurs when a hexamer of triskelions is formed with crossing over of the leg portions of the triskelions.<ref name="crowther" /> Rather than involving crossing over, simple packing refers to side by side packing of the leg portions of the triskelions (Figure 2).<ref name="crowther" />

Individual clathrin molecules can assemble into cage-like structures. When individual clathrin molecules come together, a lattice is formed where each lattice point is associated with the center of a triskelion (from the Greek word meaning “a three legged structure”) (Figure 1).<ref name="harrison" />

[[FirstGlance/Virus_Capsids_and_Other_Large_Assemblies#Clathrin_Coat|Clathrin Coat]] shows a small, empty clathrin coat assembly of triskelions from an electron microscopic structure, [[3iyv]].

According to Crowther, R.A., clathrin vesicles are constructed from twelve pentagonal units plus a number of hexagonal units, with the number of hexagons increasing for larger clathrin coats.<ref name="crowther">PMID: 7328122 </ref> The term “cage” can be used to refer to empty shells composed only of clathrin while the term “coat” refers to clathrin in addition to associated proteins and vesicles.<ref name="crowther" /> Clathrin lattices are formed under optimal conditions of physiological pH (6.0-6.5) and in the presence of Calcium or Magnesium ions.<ref name="crowther" /> Various packing models have been investigated as to how the individual triskelion legs assemble into clathrin cages.<ref name="crowther" /> As mentioned above, the center of the triskelion legs occurs at each vertex of the cage, meaning pentagons, heptagons, and hexagons can be formed.<ref name="crowther" /> In the individual triskelion legs, the bend in the leg is 160 Angstroms from the vertex, giving rise to two models of packing: simple side-by-side packing or a cross-over type of packing (Figure 2).<ref name="crowther" /> Cross-over packing occurs when a hexamer of triskelions is formed with crossing over of the leg portions of the triskelions.<ref name="crowther" /> Rather than involving crossing over, simple packing refers to side by side packing of the leg portions of the triskelions (Figure 2).<ref name="crowther" />

[[Image:LaatticeStructure2.png |frame| '''Figure 2'''. Models of the various packing models of triskelion components of clathrin.<ref name="crowther" /> Each vertex of the polygon is made of a triskelion and the legs of the triskelion run along the edges. (a) Simple side-by-side packing model of the legs in which there is no overlap and a hexamer is formed. (b) Model of cross-over packing of the triskelion legs forming a hexamer and (c) Similar model as (b) except a pentamer is formed by the triskelions with crossing over of the leg portions.<ref name="crowther" />]]

Clathrin JMU: Difference between revisions

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 12: / Line 12: @@
 ===Lattice===
-Individual clathrin molecules can assemble into cage-like structures. When individual clathrin molecules come together, a lattice is formed where each lattice point is associated with the center of a triskelion (from the Greek word meaning “a three legged structure”) (Figure 1).<ref name="harrison" /> According to Crowther, R.A., clathrin vesicles are constructed from twelve pentagonal units plus a number of hexagonal units, with the number of hexagons increasing for larger clathrin coats.<ref name="crowther">PMID: 7328122 </ref> The term “cage” can be used to refer to empty shells composed only of clathrin while the term “coat” refers to clathrin in addition to associated proteins and vesicles.<ref name="crowther" /> Clathrin lattices are formed under optimal conditions of physiological pH (6.0-6.5) and in the presence of Calcium or Magnesium ions.<ref name="crowther" /> Various packing models have been investigated as to how the individual triskelion legs assemble into clathrin cages.<ref name="crowther" /> As mentioned above, the center of the triskelion legs occurs at each vertex of the cage, meaning pentagons, heptagons, and hexagons can be formed.<ref name="crowther" /> In the individual triskelion legs, the bend in the leg is 160 Angstroms from the vertex, giving rise to two models of packing: simple side-by-side packing or a cross-over type of packing (Figure 2).<ref name="crowther" /> Cross-over packing occurs when a hexamer of triskelions is formed with crossing over of the leg portions of the triskelions.<ref name="crowther" /> Rather than involving crossing over, simple packing refers to side by side packing of the leg portions of the triskelions (Figure 2).<ref name="crowther" />
+Individual clathrin molecules can assemble into cage-like structures. When individual clathrin molecules come together, a lattice is formed where each lattice point is associated with the center of a triskelion (from the Greek word meaning “a three legged structure”) (Figure 1).<ref name="harrison" />
+[[FirstGlance/Virus_Capsids_and_Other_Large_Assemblies#Clathrin_Coat|Clathrin Coat]] shows a small, empty clathrin coat assembly of triskelions from an electron microscopic structure, [[3iyv]].
+According to Crowther, R.A., clathrin vesicles are constructed from twelve pentagonal units plus a number of hexagonal units, with the number of hexagons increasing for larger clathrin coats.<ref name="crowther">PMID: 7328122 </ref> The term “cage” can be used to refer to empty shells composed only of clathrin while the term “coat” refers to clathrin in addition to associated proteins and vesicles.<ref name="crowther" /> Clathrin lattices are formed under optimal conditions of physiological pH (6.0-6.5) and in the presence of Calcium or Magnesium ions.<ref name="crowther" /> Various packing models have been investigated as to how the individual triskelion legs assemble into clathrin cages.<ref name="crowther" /> As mentioned above, the center of the triskelion legs occurs at each vertex of the cage, meaning pentagons, heptagons, and hexagons can be formed.<ref name="crowther" /> In the individual triskelion legs, the bend in the leg is 160 Angstroms from the vertex, giving rise to two models of packing: simple side-by-side packing or a cross-over type of packing (Figure 2).<ref name="crowther" /> Cross-over packing occurs when a hexamer of triskelions is formed with crossing over of the leg portions of the triskelions.<ref name="crowther" /> Rather than involving crossing over, simple packing refers to side by side packing of the leg portions of the triskelions (Figure 2).<ref name="crowther" />
 [[Image:LaatticeStructure2.png |frame| '''Figure 2'''. Models of the various packing models of triskelion components of clathrin.<ref name="crowther" /> Each vertex of the polygon is made of a triskelion and the legs of the triskelion run along the edges. (a) Simple side-by-side packing model of the legs in which there is no overlap and a hexamer is formed. (b) Model of cross-over packing of the triskelion legs forming a hexamer and (c) Similar model as (b) except a pentamer is formed by the triskelions with crossing over of the leg portions.<ref name="crowther" />]]