4z43: Difference between revisions

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'''~~Unreleased structure~~'''

==Crystal structure of Tryptophan 7-halogenase (PrnA) Mutant E450K==

~~The entry~~ 4z43 is ~~ON HOLD~~

== Structural highlights ==

<table><tr><td colspan='2'>[[4z43]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z43 OCA]. For a guided tour on the structure components use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z43 FirstGlance].

~~Authors~~: ~~Levy~~, C.W.

</td></tr><tr id='ligand'><td class="sblockLbl">[[Ligand|Ligands:]]</td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>

<tr id='activity'><td class="sblockLbl">Activity:</td><td class="sblockDat">[http://en.wikipedia.org/wiki/Tryptophan_7-halogenase Tryptophan 7-halogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.19.9 1.14.19.9] </td></tr>

~~Description~~: ~~Crystal structure~~ of ~~Tryptophan~~ 7-~~halogenase~~ (~~PrnA~~) ~~Mutant E450K~~

<tr id='resources'><td class="sblockLbl">Resources:</td><td class="sblockDat">[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z43 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z43 OCA], [http://pdbe.org/4z43 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z43 RCSB], [http://www.ebi.ac.uk/pdbsum/4z43 PDBsum]</td></tr>

[[Category: ~~Unreleased Structures~~]]

</table>

[[Category: Levy, C.W]]

== Function ==

[[http://www.uniprot.org/uniprot/PRNA_PSEFL PRNA_PSEFL]] Involved in the biosynthesis of the antifungal antibiotic pyrrolnitrin. Catalyze the chlorination of tryptophan (Trp) at C7 position to yield 7-chloro-L-tryptophan (7-CLT). The reaction between FADH2, Cl-, and O2 generates the powerful oxidant HOCl, which is presumed to carry out the chlorination reaction. The reaction of HOCl with the active site Lys-79 generates a lysine chloramine, which plays a key role in directing regiospecific chlorination of substrate in this important class of biosynthetic enzymes. It is also able to use bromide ions to generate monobrominated Trp.<ref>PMID:10941070</ref> <ref>PMID:9172332</ref> <ref>PMID:9537395</ref>

== References ==

__TOC__

</StructureSection>

[[Category: Tryptophan 7-halogenase]]

[[Category: Levy, C W]]

[[Category: Oxidoreductase]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
+==Crystal structure of Tryptophan 7-halogenase (PrnA) Mutant E450K==
+<StructureSection load='4z43' size='340' side='right' caption='[[4z43]], [[Resolution|resolution]] 2.29&Aring;' scene=''>
-The entry 4z43 is ON HOLD
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4z43]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z43 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z43 FirstGlance]. <br>
-Authors: Levy, C.W.
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tryptophan_7-halogenase Tryptophan 7-halogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.19.9 1.14.19.9] </span></td></tr>
-Description: Crystal structure of Tryptophan 7-halogenase (PrnA) Mutant E450K
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z43 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z43 OCA], [http://pdbe.org/4z43 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z43 RCSB], [http://www.ebi.ac.uk/pdbsum/4z43 PDBsum]</span></td></tr>
-[[Category: Unreleased Structures]]
+</table>
-[[Category: Levy, C.W]]
+== Function ==
+[[http://www.uniprot.org/uniprot/PRNA_PSEFL PRNA_PSEFL]] Involved in the biosynthesis of the antifungal antibiotic pyrrolnitrin. Catalyze the chlorination of tryptophan (Trp) at C7 position to yield 7-chloro-L-tryptophan (7-CLT). The reaction between FADH2, Cl-, and O2 generates the powerful oxidant HOCl, which is presumed to carry out the chlorination reaction. The reaction of HOCl with the active site Lys-79 generates a lysine chloramine, which plays a key role in directing regiospecific chlorination of substrate in this important class of biosynthetic enzymes. It is also able to use bromide ions to generate monobrominated Trp.<ref>PMID:10941070</ref> <ref>PMID:9172332</ref> <ref>PMID:9537395</ref>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Tryptophan 7-halogenase]]
+[[Category: Levy, C W]]
+[[Category: Oxidoreductase]]