1bos: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1bos.gif|left|200px]] | [[Image:1bos.gif|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_1bos", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
| | or leave the SCENE parameter empty for the default display. | ||
| | --> | ||
{{STRUCTURE_1bos| PDB=1bos | SCENE= }} | |||
}} | |||
'''SHIGA-LIKE TOXIN COMPLEXED WITH ITS RECEPTOR''' | '''SHIGA-LIKE TOXIN COMPLEXED WITH ITS RECEPTOR''' | ||
| Line 32: | Line 29: | ||
[[Category: Ling, H.]] | [[Category: Ling, H.]] | ||
[[Category: Read, R J.]] | [[Category: Read, R J.]] | ||
[[Category: | [[Category: Ob-fold]] | ||
[[Category: | [[Category: Protein-carbohydrate recognition]] | ||
[[Category: | [[Category: Receptor binding]] | ||
[[Category: | [[Category: Toxin]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 11:46:38 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 08:46, 2 May 2008
SHIGA-LIKE TOXIN COMPLEXED WITH ITS RECEPTOR
Overview
Shiga-like toxin I (SLT-I) is a virulence factor of Escherichia coli strains that cause disease in humans. Like other members of the Shiga toxin family, it consists of an enzymatic (A) subunit and five copies of a binding subunit (the B-pentamer). The B-pentamer binds to a specific glycolipid, globotriaosylceramide (Gb3), on the surface of target cells and thereby plays a crucial role in the entry of the toxin. Here we present the crystal structure at 2.8 A resolution of the SLT-I B-pentamer complexed with an analogue of the Gb3 trisaccharide. The structure reveals a surprising density of binding sites, with three trisaccharide molecules bound to each B-subunit monomer of 69 residues. All 15 trisaccharides bind to one side of the B-pentamer, providing further evidence that this side faces the cell membrane. The structural model is consistent with data from site-directed mutagenesis and binding of carbohydrate analogues, and allows the rational design of therapeutic Gb3 analogues that block the attachment of toxin to cells.
About this Structure
1BOS is a Single protein structure of sequence from Bacteriophage h30, bacteriophage h19b. Full crystallographic information is available from OCA.
Reference
Structure of the shiga-like toxin I B-pentamer complexed with an analogue of its receptor Gb3., Ling H, Boodhoo A, Hazes B, Cummings MD, Armstrong GD, Brunton JL, Read RJ, Biochemistry. 1998 Feb 17;37(7):1777-88. PMID:9485303 Page seeded by OCA on Fri May 2 11:46:38 2008
