Eukaryotic initiation factor

Function

Eukaryotic initiation factors (EIF) are involved in the initiation of protein translation. They form a complex with the small subunit of the ribosome and Met-tRNA which scans the mRNA and recognizes the initiation codon AUG. For details on EIF4 see C-terminal portion of human eIF4GI.

Disease

Overexpression of individual subunits of eIF3 may cause malignant transformation .

Structural insights

EIF domains include the N terminal domain (NTD), C terminal domain (CTD), RNA recognition domain, middle domain, PAZ domain of EIF2C and a protein-protein interaction domain (PCI) of EIF3.

3D structures of eukaryotic initiation factor

Updated on 20-January-2016

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• EIF1
  • 2if1, 1d7q – hEIF1 – human – NMR
    
  • 2ogh - yEIF1 – NMR
    
  • 3j80, 3j81 – EIF1A in 40S ribosome – Kluyveromyces lactis – cryo EM
    
  • 4kzx, 4kzy, 4kzz – EIF1A in 40S ribosome – rabbit – cryo EM
    
  • 3zjy – hEIF1A + importin 13 + RanGTP
    
• EIF2
  • 1kl9, 1q8k – hEIF2A subunit 1
    
  • 1q8x – hEIF2A - NMR
    
  • 1si2, 1si3, 4ei3, 4f3t – hEIF2C PAZ domain + DNA
    
  • 3luk - hEIF2C MID domain
    
  • 3lud, 3lug, 3luh, 3luj, 3qx8, 3qx9 - hEIF2C MID domain + nucleotide
    
  • 1q46 – yEIF2A – yeast
    
  • 3wj9 - EIF2A – fission yeast
    
  • 2a19, 2a1a – yEIF2A (mutant) + interferon-induced protein kinase
    
  • 3ecs – hEIF2B
• EIF3
  • 4k51, 4u1d - yEIF3A PCI domain
    
  • 2nlw - hEIF3B RNA recognition domain – NMR
    
  • 3ns5, 3ns6 - yEIF3B RNA recognition domain
    
  • 4u1f - yEIF3B middle domain
    
  • 2krb - hEIF3B RNA recognition domain + hEIF3J peptide
    
  • 4nox – EIF3B – Chaetomium thermophilum
    
  • 2cq0 – hEIF3G RNA recognition domain – NMR
    
  • 2mjc - hEIF3G residues 153-176 – NMR
    
  • 3zwl - yEIF3I + EIF3B CTD
    
  • 4u1c - yEIF3A PCI + yEIF3C PCI
    
  • 4u1e - yEIF3B CTD + yEIF3I + yEIF3G NTD
    
  • 3bpj – hEIF3J
    
  • 1rz4 – hEIF3K
    
  • 3j8b, 3j8c - hEIF3A + hEIF3C + hEIF3E + hEIF3F + hEIF3H + hEIF3K + hEIF3L + hEIF3M – cryo EM
    
• EIF4
  • 1fuk – yEIF4A CTD
    
  • 1fuu - yEIF4A mRNA helicase domain
    
  • 2g9n, 3bor – hEIF4A DEAD domain
    
  • 1wi8, 2j76 – hEIF4B RNA recognition domain - NMR
    
  • 2gpq, 3tf2 – hEIF4E
    
  • 2wmc - EIF4E - pea
    
  • 2idr – wEIF4E – wheat
    
  • 3l6a - hEIF4GII CTD
    
  • 1ug3 – hEIF4GI CTD (mutant)
    
  • 1hu3, 3d3m, 4iul – hEIF4GII middle domain
    
  • 2dng – hEIF4H RNA recognition domain – NMR
    
• EIF4 complexes
  • 3ex7, 2xb2 – hEIF4A + MAGO homolog + RNA-binding protein 8A + CASC3 + RNA
    
  • 2zu6, 3eiq – hEIF4A + programmed cell death protein
    
  • 4c9b - hEIF4A + CWC22
    
  • 2jgc - hEIF4E + 4E-BP1 peptide
    
  • 2jgb - hEIF4E + nucleotide + 4E-BP1 peptide
    
  • 4tpw, 4tqb, 4tqc - hEIF4E + nucleotide + inhibitor
    
  • 4bea - hEIF4E + stapled peptide
    
  • 1ejh – mEIF4E + EIF4G peptide + GDP – mouse
    
  • 1ej1, 1l8b, 4b6u, 4b6v - mEIF4E + nucleotide
    
  • 2idv - wEIF4E (mutant) + GDP
    
  • 1ipb, 1ipc - hEIF4E + nucleotide
    
  • 4dt6, 4dum – hEIF4E + inhibitor
    
  • 2w97, 4aza – hEIF4E + hEIF4G peptide
    
  • 1wkw - hEIF4E + EIF4E-binding protein peptide + GDP
    
  • 2v8w, 2v8x, 2v8y, 3u7x - hEIF4E + EIF4E-binding protein peptide
    
  • 3am7 - hEIF4E + EIF4E-binding protein peptide + nucleotide
    
  • 1ej4 - mEIF4E + EIF4E-binding protein peptide
    
  • 3hxg, 3hxi - EIF4E + EIF4E-binding protein peptide + GDP – Schistosoma mansoni
    
  • 3m93, 3m94 - EIF4E + EIF4E-binding protein peptide + nucleotide – Pig roundworm
    
  • 1ap8 – yEIF4E + GDP – NMR
    
  • 1rf8 - yEIF4E + yEIF4GI peptide + GDP
    
  • 2vso, 2vsx - yEIF4E + yEIF4F
    
  • 4axg - DmEIF4E + protein CUP – Drosophila melanogaster
    
  • 4ue8, 4uea, 4ueb, 4ued - DmEIF4E + 4E-binding protein
    
  • 4ue9 - DmEIF4E + 4E transporter
    
  • 4uec - DmEIF4E + DmEIF4G
    
• EIF5
  • 1eif, 2eif – EIF5A – Methanocaldococcus janaschii
    
  • 1x6o – EIF5A – Leishmania braziliensis
    
  • 1xtd - EIF5A – Leishmania mexicana
    
  • 3hks – EIF5A – Arabidopsis thaliana
    
  • 3cpf - hEIF
    
  • 2ful – yEIF5 CTD
    
  • 2iu1 - hEIF5 CTD
    
  • 2g2k – hEIF5 NTD (mutant) – NMR
    
  • 2e9h – hEIF5 – NMR
    
  • 3cpf – hEIF5A
    
  • 3er0 - yEIF5A
    
  • 3wbi, 3wbj, 4n3s – yEIF5B
    
  • 3wbk – yEIF5B + yEIF1A
    
  • 4ncf – yEIF5B + nucleotide
    
• EIF6
  • 1g62 – yEIF6
    
  • 2x7n – yEIF6S + sarcin-ricin loop + ribosomal protein L23 + ribosomal protein L24
    
  • 3j2i – yEIF6 + proliferation-associated protein 2G4 – cryo EM
    

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Michal Harel, Alexander Berchansky, Joel L. Sussman