5ayv

Crystal structure of archaeal ketopantoate reductase complexed with coenzyme A and 2-oxopantoate

spinqualitylabelsall models 5ayv, resolution 1.65Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5ayv is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ACT, COA, KPL, MPD, NAP Activity: 2-dehydropantoate 2-reductase, with EC number 1.1.1.169 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum Function [Q5JGC2_THEKO] Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.[RuleBase:RU362068] Publication Abstract from PubMed Coenzyme A (CoA) plays essential roles in a variety of metabolic pathways in all three domains of life. The biosynthesis pathway of CoA is strictly regulated by feedback inhibition. In bacteria and eukaryotes, pantothenate kinase is the target of feedback inhibition by CoA. Recent biochemical studies have identified ketopantoate reductase (KPR), which catalyzes the NAD(P)H-dependent reduction of 2-oxopantoate to pantoate, as a target of the feedback inhibition by CoA in archaea. However the mechanism for recognition of CoA by KPR is still unknown. Here we report the crystal structure of KPR from Thermococcus kodakarensis in complex with CoA and 2-oxopantoate. CoA occupies the binding site of NAD(P)H, explaining the competitive inhibition by CoA. Our structure reveals a disulfide bond between CoA and Cys84 that indicates an irreversible inhibition upon binding of CoA. The structure also suggests the cooperative binding of CoA and 2-oxopantoate that triggers a conformational closure and seems to facilitate the disulfide bond formation. Our findings provide novel insights into the mechanism that regulates biosynthesis of CoA in archaea. This article is protected by copyright. All rights reserved. Crystal structure of archaeal ketopantoate reductase complexed with coenzyme A and 2-oxopantoate provides structural insights into feedback regulation.,Aikawa Y, Nishitani Y, Tomita H, Atomi H, Miki K Proteins. 2016 Jan 12. doi: 10.1002/prot.24984. PMID:26757028 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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