Sandbox Reserved 1125: Difference between revisions
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=== Pocket interaction === | === Pocket interaction === | ||
==== Ca2+ ==== | ==== Ca2+ ==== | ||
[[Image:CA_pocket_interaction.gif | thumb|CA pocket interaction]] | [[Image:CA_pocket_interaction.gif | thumb|CA pocket interaction]]This enzyme binds 3 CA ions per subunit. | ||
The active site of the enzyme is composed by two Gly residues (169 and 171) next to two Asp residues (137 and 173) | The active site of the enzyme is composed by two Gly residues (169 and 171) next to two Asp residues (137 and 173): <scene name='71/719866/Ca2_interactions/1'>see on the structure</scene> | ||
<font color='red'>maintain a Zn2+ atom coordinated with three water molecules. One of them is as well bound to the Glu residue thanks to a hydrogen bond. The second Zn2+ atom is not involved in the active site. At first, the Gly 206 residue of the substrate binds the active site thanks to the Zn2+ atom. When it binds it takes the place of unstable water molecules and establishes stabilizing interactions with the active site thanks to its C terminal part. Then, the Ala 182 residue of the enzyme makes a hydrogen bond with the NH group of the substrate: this allows the substrate to enter the cavity of the catalytic site. The rest of the protein is stabilized by 4 hydrogen bonds with the amino acid located in the cavity.</font> | <font color='red'>maintain a Zn2+ atom coordinated with three water molecules. One of them is as well bound to the Glu residue thanks to a hydrogen bond. The second Zn2+ atom is not involved in the active site. At first, the Gly 206 residue of the substrate binds the active site thanks to the Zn2+ atom. When it binds it takes the place of unstable water molecules and establishes stabilizing interactions with the active site thanks to its C terminal part. Then, the Ala 182 residue of the enzyme makes a hydrogen bond with the NH group of the substrate: this allows the substrate to enter the cavity of the catalytic site. The rest of the protein is stabilized by 4 hydrogen bonds with the amino acid located in the cavity.</font> | ||
