Sandbox Reserved 1125: Difference between revisions
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=== Homopexin domain === | === Homopexin domain === | ||
The hemopexin domain has two conserved cysteines that are disulfide bonded. Mutation of those cysteines to alanines <ref>PMID:8464863</ref> or reduction and alkylation destroys collagenolytic activity (K. Suzuki and H.Nagase, unpublished results).<ref name="Pdf"/> | The hemopexin domain has two conserved cysteines that are disulfide bonded. Mutation of those cysteines to alanines <ref>PMID:8464863</ref> or reduction and alkylation destroys collagenolytic activity (K. Suzuki and H.Nagase, unpublished results).<ref name="Pdf"/> | ||
[http://www.jleukbio.org/content/81/4/870.full] | |||
This domain is localized outside of the catalytic domain. It is essential for the substrate recognition of MMP-8 because this domain is removed, the protein loses its ability to cleave collagen. However, neutrophil collagenase is still able to cleave other substrate. | |||
== Mechanism == | == Mechanism == | ||
