Sandbox Reserved 1125: Difference between revisions

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=== Pocket interaction ===

==== Ca2+ ====

[[Image:CA_pocket_interaction.gif | thumb|CA pocket interaction]]This enzyme binds 3 CA ions ~~per subunit~~.

[[Image:CA_pocket_interaction.gif | thumb|CA pocket interaction]]This enzyme binds 3 Ca ions, 2 of them in the catalytic domain.

The residues involved in the Ca996 interactions are <scene name='71/719866/Ca2_interactions/1'>two Gly residues (169 and 171) next to two Asp residues (137 and 173)</scene>.

The residues involved in the Ca996 interactions (coordinate bonds) are <scene name='71/719866/Ca2_interactions/1'>two Gly residues (169 and 171) next to two Asp residues (137 and 173)</scene>.

==== Zn2+ ====

The Zn ion which is involved in the catalytic activity is the Zn999. In a publication (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC394940/?page=2) which studies the catalytic domain of MMP8 thanks to the Pro-Leu-Gly-hydroxylamine inhibitor, this ion is penta-coordinated with: His197, His201 and His207 of MMP8 and with the carbonyl and the hydroxyl oxygen of the hydroxamic acid moiety of the inhibitor.

Zn999

The Zn ion which is involved in the catalytic activity is the Zn999. In a publication (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC394940/?page=2) which studies the catalytic domain of MMP8 thanks to the Pro-Leu-Gly-hydroxylamine inhibitor, this ion is penta-coordinated with: His197, His201 and His207 of MMP8 and with the carbonyl and the hydroxyl oxygen of the hydroxamic acid moiety of the inhibitor. On this <scene name='71/719866/Zn999_interactions/2'>link</scene> you can only see the 3 His of MMP8 with the Zn999.

[[Image:ZN pocket interaction.gif | thumb|ZN pocket interaction]]

Zn998

The residues involved in the Zn interactions are one <scene name='71/719866/Zn998/1'>Asp residue (149) next to three His residues (147, 162 and 175)</scene>.

This enzyme binds 2ZN ions per subunit.

Sandbox Reserved 1125: Difference between revisions

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@@ Line 39: / Line 39: @@
 === Pocket interaction ===
 ==== Ca2+ ====
-[[Image:CA_pocket_interaction.gif | thumb|CA pocket interaction]]This enzyme binds 3 CA ions per subunit.
+[[Image:CA_pocket_interaction.gif | thumb|CA pocket interaction]]This enzyme binds 3 Ca ions, 2 of them in the catalytic domain.
-The residues involved in the Ca996 interactions are <scene name='71/719866/Ca2_interactions/1'>two Gly residues (169 and 171) next to two Asp residues (137 and 173)</scene>.
+The residues involved in the Ca996 interactions (coordinate bonds) are <scene name='71/719866/Ca2_interactions/1'>two Gly residues (169 and 171) next to two Asp residues (137 and 173)</scene>.
 ==== Zn2+ ====
-The Zn ion which is involved in the catalytic activity is the Zn999. In a publication (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC394940/?page=2) which studies the catalytic domain of MMP8 thanks to the Pro-Leu-Gly-hydroxylamine inhibitor, this ion is penta-coordinated with: His197, His201 and His207 of MMP8 and with the carbonyl and the hydroxyl oxygen of the hydroxamic acid moiety of the inhibitor.
+Zn999
+The Zn ion which is involved in the catalytic activity is the Zn999. In a publication (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC394940/?page=2) which studies the catalytic domain of MMP8 thanks to the Pro-Leu-Gly-hydroxylamine inhibitor, this ion is penta-coordinated with: His197, His201 and His207 of MMP8 and with the carbonyl and the hydroxyl oxygen of the hydroxamic acid moiety of the inhibitor. On this <scene name='71/719866/Zn999_interactions/2'>link</scene> you can only see the 3 His of MMP8 with the Zn999.
 [[Image:ZN pocket interaction.gif | thumb|ZN pocket interaction]]
+Zn998
 The residues involved in the Zn interactions are one <scene name='71/719866/Zn998/1'>Asp residue (149) next to three His residues (147, 162 and 175)</scene>.
 This enzyme binds 2ZN ions per subunit.