1cq0: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1cq0.jpg|left|200px]] | [[Image:1cq0.jpg|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_1cq0", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
| | or leave the SCENE parameter empty for the default display. | ||
| | --> | ||
{{STRUCTURE_1cq0| PDB=1cq0 | SCENE= }} | |||
}} | |||
'''SOLUTION STRUCTURE OF A HUMAN HYPOCRETIN-2/OREXIN-B'SOLUTION STRUCTURE OF A HUMAN HYPOCRETIN-2/OREXIN-B '''' | '''SOLUTION STRUCTURE OF A HUMAN HYPOCRETIN-2/OREXIN-B'SOLUTION STRUCTURE OF A HUMAN HYPOCRETIN-2/OREXIN-B '''' | ||
| Line 30: | Line 27: | ||
[[Category: Lee, K H.]] | [[Category: Lee, K H.]] | ||
[[Category: Lee, W.]] | [[Category: Lee, W.]] | ||
[[Category: | [[Category: Human hcrt-2/ox-b,neuropeptide]] | ||
[[Category: | [[Category: Nmr]] | ||
[[Category: | [[Category: Obesity]] | ||
[[Category: | [[Category: Solution structure]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:59:27 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 09:59, 2 May 2008
SOLUTION STRUCTURE OF A HUMAN HYPOCRETIN-2/OREXIN-B'SOLUTION STRUCTURE OF A HUMAN HYPOCRETIN-2/OREXIN-B '
Overview
Orexin-A and orexin-B (also called hypocretin-1 and hypocretin-2, respectively) are novel hypothalamic neuropeptides encoded by a single mRNA transcript; they stimulate food intake. We have determined the three-dimensional solution structure of human hypocretin-2/orexin-B using two-dimensional 1H-NMR data and dynamical simulated annealing calculations. On the basis of NOEs, 3JHNalpha coupling constants and hydrogen-deuterium exchange rates together with chemical shift indices, human hypocretin-2/orexin-B was deduced to consist of two alpha-helices connected with a short linker in both H2O and 30% trifluoroethanol solutions. The helical axis of helix I is oriented about 60-80 degrees relative to helix II. Hybrid distance geometry and simulated-annealing protocols were used to generate an ensemble of 30 structures with no constraint violations greater than 0.03 nm for distances and 3 degrees for angles. In addition, human hypocretin-2/orexin-B shares a similar secondary-structural motif with human neuropeptide Y. This result can form the basis for further study on ligand-receptor recognition of human orexin receptors.
About this Structure
1CQ0 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Solution structure of a new hypothalamic neuropeptide, human hypocretin-2/orexin-B., Lee JH, Bang E, Chae KJ, Kim JY, Lee DW, Lee W, Eur J Biochem. 1999 Dec;266(3):831-9. PMID:10583376 Page seeded by OCA on Fri May 2 12:59:27 2008
